Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7.3, Problem 2COMQ
In mapping experiments, ______ strains are conjugated to
a.
b. Hfr, times of entry
c.
d. Hfr, expression levels
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If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene?
A.
insert the known gene into a vector and use the vector to insert the known gene into the other organism
B.
treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis
C.
create a hybrid of the two organisms by breeding them and check for mutations
D.
create labeled DNA probes from the known gene and use them to search the genome of the other organism
a. How do scientists apply the concept of linkage disequilibrium to identify disease alleles?
b. Which specific phrase is used when such markers are identified by restriction endonucleases and a particular set of DNA fragments is generated?
A bacterial transformation is performed with a donor strain that is resistant to four drugs, A, B, C, and D, and a recipient strain that is sensitive to all four drugs. The resulting recipient cell population is divided and plated onmedia containing various combinations of the drugs. The following table shows the results.a. One of the genes is distant from the other three, which appear to be closely linked. Which is the distant gene? b. What is the likely order of the three closely linked genes
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- In DNA-DNA Hybridization, sequences from two separate organisms that show partial hybridization suggest _______. a) They are related b) suggest they are not related c)suggest that they are the same organismarrow_forwardExplain the role of the gene that is the selectable marker gene in this experiment.arrow_forwardA donor strain of bacteria with alleles a+ b+ c+ is infected with phages to map the donor chromosome using generalized transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a− b− c−. Bacteria with the a+ allele are selected, and the percentage of cells with cotransduced b+ and c+ alleles are recorded. Donor Recipient Selected allele Cells with cotransduced allele (%) a+ b+ c+ a− b− c− a+ 25 b+ a+ 3 c+ Is gene b or gene c closer to gene a? Explain your reasoning.arrow_forward
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