Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7.3, Problem 1COMQ
With regard to conjugation, a key difference between
a. is unable to conjugate.
b. transfers a plasmid to the recipient cell.
c. transfers a portion of the bacterial chromosome to the recipient cell.
d. becomes an
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You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector.
a) How should this plasmid vector be constructed?
b) Also describe the function of the components.
With regard to conjugation, a key difference between F+ and Hfrcells is that an Hfr cella. is unable to conjugate.b. transfers a plasmid to the recipient cell.c. transfers a portion of the bacterial chromosome to the recipient cell.d. becomes an F− cell after conjugation.
Which of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forwardConjugation involves which of the following: a. transfer of plasmids among bacteria b. sexual reproduction of Eukaryotes c. uptake of free DNA from the environment d. integration of a virus into a chromosomearrow_forwardWhich of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forward
- Explain the importance of the following features in conjugating donor bacteria: a. the origin of transfer b. the conjugation pilus c. homologous recombination d. the relaxosome e. relaxase f. T strand DNA g. pillin proteinarrow_forwardinvolved in the transfer of multiple drug resistance from one cell to another? * a.Transposition b. Conjugation with a cell with chromosomal drug resistance appears in the genome of a bacteriophage that has infected it. c. Transformation of chromosomal genes d. Conjugation with a cell with a free plasmid carrying drug resistancearrow_forwardWhat would be the result if an organism’s telomerase were mutated and nonfunctional? a. No DNA replication would take place. b. The DNA polymerase enzyme would stall at the telomere. c. Chromosomes would shorten with each new generation. d. RNA primers could not be removed.arrow_forward
- Which of the following statements is false? a. Plasmids can be exchanged between different species of bacteria. b. The blue circle represents a small chromosome. c. The blue circle is a plasmid that contains genes that confer resistance to specific antiobiotics. d. The blue circle represents a double-stranded molecule of DNA.arrow_forwardWhat carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardSmall circular molecules of DNA in bacteria ____. a. result from the activity of restriction enzymes b. are plasmids c. cannot survive outside of the cell d. are eventually degraded e. are DNA fragments from their main chromosomearrow_forward
- The polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forwardFollowing a CRISPR mediated DNA double-strand break, how can the DNA be repaired by the cell? Choose all that apply. A. Base pairing between complementary sticky sides. B. Nonhomologous end joining. C. Homology directed repair. D. Crossing over between nonhomologous chromosomes.arrow_forwardA strain with the ability to conjugate is mixed with a recipient strain. Recipients are noted to become recombinant but F-. This indicates. a. unsuccessful completion of conjugation b. the donor strain has a defective plasmid c. the donor strain has a plasmid integrated in the chromosome d. the recipient has a defective recA genearrow_forward
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