Concept explainers
A.
To explain: The single operator site that is most important for repression
Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for
B.
To explain: Whether the combinations of operator sites substantially increase repression by the dimeric repressor and the combination of operator sites substantially increase repression by the tetrameric repressor. The reason as to why the two repressors behave differently.
Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for metabolism of lactose. The Lac operon includes operator, promoter, repressor and the genes that code for three enzymes required for conversation of lactose to glucose.
C.
To explain: The wild type repressor binds strongly when O3 is present with O1.
Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for metabolism of lactose. The Lac operon includes operator, promoter, repressor and the genes that code for three enzymes required for conversation of lactose to glucose.
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Molecular Biology of the Cell (Sixth Edition)
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