Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
6th Edition
ISBN: 9780815344322
Author: Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher: W. W. Norton & Company
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Chapter 8, Problem 19P

A.

Summary Introduction

To explain: The single operator site that is most important for repression

Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for metabolism of lactose. The Lac operon includes operator, promoter, repressor and the genes that code for three enzymes required for conversation of lactose to glucose.

B.

Summary Introduction

To explain: Whether the combinations of operator sites substantially increase repression by the dimeric repressor and the combination of operator sites substantially increase repression by the tetrameric repressor. The reason as to why the two repressors behave differently.

Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for metabolism of lactose. The Lac operon includes operator, promoter, repressor and the genes that code for three enzymes required for conversation of lactose to glucose.

C.

Summary Introduction

To explain: The wild type repressor binds strongly when O3 is present with O1.

Concept introduction: Prokaryotes regulate their genes using operons. Operons control transcription of multiple genes. Lac operon controls the genes that produce enzymes necessary for metabolism of lactose. The Lac operon includes operator, promoter, repressor and the genes that code for three enzymes required for conversation of lactose to glucose.

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