MICROBIOLOGY W/DISEASES (SPIRAL) >CI<
17th Edition
ISBN: 9781323471661
Author: BAUMAN
Publisher: PEARSON C
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Textbook Question
Chapter 8, Problem 1CM
Using the following terms, fill in the following concept map that describes recombinant DNA technology. You can also complete this and other concept maps online by going to the MasteringMicrobiology Study Area.
Blunt ends
DNA probes
Electroporation
Fluorescent
Injection
Mutagens
Plasmids
Protoplast fusion
Radioactive
Restriction enzymes
Reverse transcriptase
Southern biota
Transposons
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Chapter 8 Solutions
MICROBIOLOGY W/DISEASES (SPIRAL) >CI<
Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Prob. 2TMWCh. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Prob. 5TMWCh. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MC
Ch. 8 - Prob. 6MCCh. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Modified True/False 1. ________ Restriction...Ch. 8 - Modified True/False 2. ________ Restriction...Ch. 8 - Prob. 3MTFCh. 8 - Prob. 4MTFCh. 8 - Prob. 5MTFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - CRITICAL THINKING 2 A cancer-inducing virus,...Ch. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - Prob. 4CTCh. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - Prob. 7CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwardEnzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonucleasearrow_forwardAn viral enzyme that can be used to create complementary cDNA is DNA polymerase. reverse transcriptase. restriction endonuclease. DNA ligase. mutase.arrow_forward
- Which is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardBased on the image below, answer the following questions: Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below. The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)arrow_forwardFluorescently-labeled dideoxynucleotides are used in _____. Group of answer choices:" cDNA libraries DNA sequencing genomic DNA libraries PCRarrow_forward
- Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forwardWhich bacterial enzyme removes the primers?a. Primaseb. DNA polymerase Ic. DNA polymerase IIId. Ligasearrow_forwardwrite in one or two sentence for each please. Define the following terms:a. biotechnology: ____________________________________________________________b.genetic engineering: ________________________________________________________c. sticky ends: ______________________________________________________________d. plasmid: ________________________________________________________________e. recombinant DNA: _________________________________________________________f. genetic marker: ___________________________________________________________g. transgenic organism: _______________________________________________________h. clone: __________________________________________________________________i. vector: ________________________________________________________________arrow_forward
- ________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymesarrow_forwardFor the analysis of plasmid DNA samples, ________ that recognize, bind to and cleave DNA at specific nucleotide sequences are used. Question options: primase restriction enzyme. DNA polymerase I. reverse transcriptase.arrow_forwardWhich of the following is not denatured by heating steps during PCR?a) DNA polb) RNA polc) Restriction enzymesd) DNA ligasearrow_forward
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