Concept explainers
The diagram below shows a DNA duplex. The template strand is identified, as is the location of the
Assume this region contains a gene transcribed in a bacterium. Identify the location of promoter consensus sequences and of the transcription termination sequence.
Assume this region contains a gene transcribed to form mRNA in a eukaryote. Identify the location of the most common promoter consensus sequences.
If this region is a eukaryotic gene transcribed by RNA polymerase III, where are the promoter consensus sequences located?
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Chapter 8 Solutions
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- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. There are NO sites where multiple enzymes cut. How many base pairs long is the plasmid? 3kb 2kb 4kb 5kbarrow_forwardThe image shows a replication fork with template DNA strands, new DNA strands, and some replication proteins. Label the mage by moving the terms or descriptive phrases to the appropriate targets. lagging strand (or Okazaki fragment) Answer Bank protein that synthesizes RNA primer DnaB helicase DNA gyrase (topoisomerase)arrow_forwardIndicate the proteins involved in the following steps of DNA replication in E. coli a. ___________ initiator of replication by binding to the origin of replication of the prokaryote ___________ a dimer that unzips the DNA helix ___________ an enzyme responsible for relieving positive supercoils ahead of replication fork ___________ maintains DNA in single-stranded state ___________ primary replicating enzyme ___________ synthesizes RNA primers The resulting gap by removal of RNA primer is filled in by ___________. h. the resulting nick is sealed by _____________.arrow_forward
- U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. PscI & GsuI ______________________________________________________________ ScaI, PdmI & BsaXI ______________________________________________________________ ScaI, SspI & EheI ______________________________________________________________arrow_forwardplease dont give hand writting solution pleasearrow_forwardThe bacterial primase enzyme synthesizes _______________________. a short DNA primer using RNA as a template a short protein primer using RNA as a template an RNA strand in absence of template DNA a short DNA primer using DNA as a template a short RNA primer using DNA as a templatearrow_forward
- The chromatogram shows fluorescent peak data from a dye-terminating nucleotide-sequencing reaction. The peaks are shown with shortest fragment on the left to longer fragments on the right. T •C A Select the DNA sequence that matches the data. 5-ТАТAСТТАСGAAGT-3' 5'-GTCCTACGGACGCG–3' 5'-ATATGAATGCTTCA–3' 5'-TGAAGCATTCATAT–3' 5-АСТТCGTAAGTATA-3'arrow_forwardName the bacterial enzyme that takes over new strand synthesis in replication after the primer is formed. Please include any number that is applicable. DNA polymerase omega DNA polymerase II DNA Polymerase epsilon DNA polymerase delta O DNA Polymerase Iarrow_forwardGive typing answer with explanation and conclusionarrow_forward
- Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. What is the smallest fragment size that the NcoI/EcoRI double digest produces?arrow_forwardIn the boxes below, complete the Original DNA, DNA Copy and/or RNAarrow_forwardA Sanger product of the sequencing of a template DNA is presented +ddTTP +ddATP +ddCTP +ddGTP Mononucleotide Pentanucleotide Trinucleotide Dinucleotide 11-nucleotide Hexanucleotide Octanucleotide Tetranucleotide 16-nucleotide Decanucleotide Nonanucleotide Heptanucleotide 17-nucleotide 15-nucleotide 13-nucleotide 12-nucleotide 18-nucleotide 20-nucleotide 14-nucleotide 19-nucleotide 21-nucleotide 1. Determine the sequence of template DNA 2. Determine the mRNA sequence from this template DNA 3. Determine the the sequence of protein product derived from that DNAarrow_forward
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