The reason that it is not possible to predict whether 14 C and 13 C are present in the white circular representations of atoms as given in the figure. Introduction: One of the techniques of detecting the position of atoms and the structure and function of a molecule is based on isotope labeling. It involves both radioactive isotope labeling in which the presence of labels is checked by measuring the amount of radiation emitted from the radioactive isotope upon metabolism . Nonradioactive labeling is also called stable labeling. This is used in the NMR technique to deduce the structure of a protein or other biomolecules depending upon the resonance of the spin energy states of the atoms present inside the molecule.

Question

Phosphoglycerate mutase transfers a phosphoryl group from C3 of 3-phosphoglycerate to C2 position to give 2-phosphoglycerate. Why is it important to have the phosphoryl group at position 2 rather than at C3? It was observed that the activity of some preparations of the enzyme could be increased if catalytic (very small) amount of 2, 3-bisphosphoglycerate was added to the enzyme. Give a possible explanation for this observation.

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Chapter 9, Problem 1Q

Summary Introduction

To explain: The reason that it is not possible to predict whether ¹⁴C and ¹³C are present in the white circular representations of atoms as given in the figure.

Introduction: One of the techniques of detecting the position of atoms and the structure and function of a molecule is based on isotope labeling. It involves both radioactive isotope labeling in which the presence of labels is checked by measuring the amount of radiation emitted from the radioactive isotope upon metabolism. Nonradioactive labeling is also called stable labeling. This is used in the NMR technique to deduce the structure of a protein or other biomolecules depending upon the resonance of the spin energy states of the atoms present inside the molecule.

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Phosphoglycerate mutase transfers a phosphoryl group from C3 of 3-phosphoglycerate to C2 position to give 2-phosphoglycerate. Why is it important to have the phosphoryl group at position 2 rather than at C3? It was observed that the activity of some preparations of the enzyme could be increased if catalytic (very small) amount of 2, 3-bisphosphoglycerate was added to the enzyme. Give a possible explanation for this observation.

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