WORLD OF CELL+MASTERING ACCESS >CUSTOM
WORLD OF CELL+MASTERING ACCESS >CUSTOM
9th Edition
ISBN: 9781323445044
Author: Hardin
Publisher: PEARSON C
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Chapter 9, Problem 9.2PS

The History of Glycolysis. Following are several historical observations that led to the elucidation of the glycolytic pathway. In each case, suggest a metabolic basis for the observed effect, and explain the significance of the observation for the elucidation of the pathway.

  1. (a) Alcoholic fermentation in yeast extracts requires a heat-labile fraction originally called zymase and a heat-stable fraction (cozymase) that is necessary for the activity of zymase.
  2. (b) Alcoholic fermentation does not take place in the absence of inorganic phosphate.
  3. (c) In the presence of iodoacetate, a known inhibitor of glycolysis, fermenting yeast extracts accumulate a doubly phosphorylated hexose.
  4. (d) In the presence of fluoride ion, another known glycolytic inhibitor, fermenting yeast extracts accumulate two phosphorylated three-carbon acids.
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