Bio

Separation of Amino Acids by Thin Layer Chromatography Lab Questions 4. Why is it necessary to run TLC in a closed container and have the chamber saturated with solventvapor? 5. Why must the spot be applied to the TLC plate above the level of development solvent? 6. What will be the result of adding too much sample to the TLC plate?

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

True or False 1. Two challenges regarding IFU are adhering with the IFU's and interpreting complex IFU. 2.  Mechanical disinfection is typically more effective than manual techniques. 3. Adding a UV disinfector to a pass-through window is NOT offer a higher level a safety for sterile processing personnel.

using sample prep aka the protocol use it to fill in the tables

Can you help me?

ISOLATE #1 Results for "Isolate #1" ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 1. Based on your results, what is the possible identification (Genus and species) of “Isolate #1"? I 9 10 11 12 3 14

GROWTH CUVRVE Can someone help me with this? (This is a question from GFP experiment I did, which I have to include in my rapport and hand in)

ISOLATE #2 Results for "Isolate #2": ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 2. Based on your results, what is the possible identification (Genus and species) of "Isolate #2"? nhes

True or false?

TRUE or FALSE only! 1. The most important step in doing a slide mount technique is fixing the sample with heat or chemicals. 2. Diluted samples from the serial dilution procedure are used for pour plating and spread plating methods which can be done simultaneously. 3. In doing aseptic techniques, it is practiced to touch the tip of the inoculating loop to test its coolness for transfer of cultures. 4. One of the important points to consider when calibrating an ocular micrometer is that its lines must properly superimpose with that of a stage microruler.

Test Description Media Indicator Incubation Results components or system design Nutrient agar Triphenol deeps with a tretrazolium concentration (TTC) can be gradient of temperature (°C) Motility Aerobes will be found growing at the surface of the tube and 37 Test Medium (MTM) sometimes along the stab line when oxygen is introduced during inoculation. added better охудen visualize the growth Obligate anaerobes will be found near the bottom of the tube. Facultative anaerobes will grow throughout the tube, but because more ATP is produced aerobically growth is heaviest at the surface. Microaerophile wil1 be found growing near the narrow region where oxygen concentration is limited. patterns. Brewer's Anaerobic Agar (BAA) Anaerobic Jar 4 Candle Jar 2. 3.

Please help! Im confused

labeling excercise in phlebotomy

I am wondering whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR? Please help me with the information including how to purchase it.

mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…

For laboratory exercise of Antibiotic susceptibility test in disk-diffusion method. What does an ‘I’ result mean in disk diffusion susceptibility test?

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.

I need help with question number 5

Please answer the questions in the grey colored box provided in the image.

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Cause and Effect Analysis describe the effect on the test procedure and explain briefly The zones of adjacent antibiotic disks overlap. Antibiotic disk cartridge was left open for more than 7 days. Antibiotic disk was placed closely at the edge of the plate.

Questions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?

Give typed full explanation

General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes   c) test tubes   d) microscope slides   2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top.   b. You notice flames coming from the tubing of your Bunsen burner

IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?

Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C) electron enzyme reagent ассeptor 1 Oxidase test Tryptic 24 – 48 hours soy agar (TSA), QxiDrop 2 Catalase Охудen test N/A Nitrate 37 reductase N/A test

Use Dimensional Analysis

Separation of Amino Acids by Thin Layer Chromatography Lab Questions 7. Why must a pencil be used for drawing the line and spotting? 8. How will the Rf value of a compound be affected if the developing solvent is allowed to run off thetop of the TLC plate (e.g. the TLC plate is allowed to remain in the developing chamber after thesolvent front has reached the top of the plate.)? Explain. Be specific. 9. Describe what an iodine vapor chamber is and explain how does it work? (Hint: Backgroundinformation – showing spots up chemically talks about two methods using Ninhydrin and Iodinecrystals)

TRUE OR FALSE only! 1. Petri dishes and test tubes are containers used in cultivating microorganisms, but it is preferable to use test tubes in dealing with liquid samples. 2. You can work alone in the laboratory if you have the consent of your instructor. 3. In dispensing the same solution, you can always reuse micropipette tips. 4. The disadvantages of using liquid media are that detection and isolation of different types of bacteria from mixed populations is difficult.

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