231L+Dyes+TEMPLATE 2023-04-18 20_33_05
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Iowa State University *
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Chemistry
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Feb 20, 2024
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NEW TEMPLATE: 231L Synthetic Organic Dyes For this experiment you will by synthesizing an organic dye. You will use absorption spectroscopy, fluorescence characterization, and TLC to identify your dye. Pre-lab Questions Procedure and Safety 1.
(10 pts) Write a bullet list outline for the procedure. 2.
(5 pts) Write a brief summary of the key safety concerns specific for this experiment. Data and Observations (Only record for dye that you synthesized.) 1.
(35 pts) Record what you are instructed to record in the procedure. In addition, while you are performing the synthesis of the dye that you chose, record any issues you had or what you could have done differently in order to get better results. There were no isssues in this lab, however I did get the wrong solution for the absorbance test. That issue was caught before getting the data though. REQUIRED DATA AND OBSERVATIONS FOR BOTH DYES (Record data for the dye that you synthesized. Obtain data for the other dye from a classmate.) 2.
(5 pts) Insert a photo of dye 1 after rotovaping (to show color of compound).
3.
(5 pts) Insert a photo of dye 2 after rotovaping (to show color of compound). (the other team did not take a picture of this) 4.
(18 pts) Insert three photos showing absorption spectrum for dye 1, fluorescein, and 9-
fluorenone. Indicate the wavelenth where maximum absorption occurred in each spectrum. Be sure to label each spectrum.
5.
(18 pts) Insert three photos showing absorption spectrum for dye 2, eosin Y, and rhodamine B. Indicate the wavelenth where maximum absorption occurred in each spectrum. Be sure to label each spectrum.
6.
(18 pts) Insert three photos showing fluorescence for dye 1, fluorescein, and 9-fluorenone. Be sure to label which photo is which compound.
7.
(18 pts) Insert three photos showing fluorescence for dye 2, eosin Y, and rhodamine B. Be sure to label which photo is which compound. (other team didn’t provide 3 photos)
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Related Questions
Not exactly sure how to fill out the table?
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Hello, please answer the following attached Chemistry question correctly and fully based upon the attached table. Please answer the "Low and High absorbance" parts. Thank you.
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This is going with the first assignment I submitted. Fingerprinting
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Chemistry
Hi, i just need some help on how to calculate the concentration of the samples? Thank you
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What, if any, impact is using drops instead of specific volumes has on the experiment?
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is one able to help? please
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Instruction:
1. Using a 25-mL glass stoppered buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/3 of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/3 of the content. Record the IR and FR of the second trial.
2. Using a 25-mL rubber stopperd buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/4of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/4 of the content. Record the IR and FR of the second trial.
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Instruction:
1. Using a 25-mL glass stoppered buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/3 of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/3 of the content. Record the IR and FR of the second trial.
2. Using a 25-mL rubber stopperd buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/4of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/4 of the content. Record the IR and FR of the second trial.
3. Complete the table.
1. Buret with Glass Stopper
TRIAL 1
TRIAL 2
Final reading
Initial reading
Volume Used
2. Buret with Rubber Stopper
TRIAL 1
TRIAL 2
Final reading
Initial reading
Volume Used
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sub= 18 help
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PART ONE: Preparation of FECNS* Solution
Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled
water and CNS into two labeled test tubes. The absorbance of the solutions must be measured
soon after adding the Fe" solution from a buret.
TABLE 1
Fe
5 ml
5S mL
Solution
Distilled Water
CNS
2 mL
Total Volume
3 ml
2 ml
10 ml
3 mL
10 ml
In this part of the experiment you are to explore what the above quote means. As a
PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the
following circumstances:
1. 1ml of 0.0020 M CNS' solution is added to the unused portion of
solution 1 in the test tube
2. One drop of 0.20 M CNS solution is added to the unused portion of
solution 2 in the test tube.
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Using the lab instructions and data table with data, please help me complete part 1 and 2 of my lab worksheet
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St. John's University
Department of Chemistry
CHE2241L Date 427/2022 8:00AM
Organic Lab Final Exam
NAME
1) The attached picture shows the structure of aluminum oxide the column
chromatography stationary phase that we used to separate fluorene from
fluorenone. Iis this compound
a) Polar
5) Nonpolar
Explain why it is polar or nonpolar. It is
because
2) Safety: Which of the following chemicals are extremely flammable:
a) diethyl ether
b) acetone
c) methanol
d) benzyl alcohol
e) All of the above except d.
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It's important to mark the solvent front with a pencil after the plate is finished being developed in order to calculate an Rf.
Group of answer choices
True
False
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3. Using the data at right:
a.
Calculate the absorbance of each sample. You will
need to express the %T values as decimals before
calculating the logarithms.
(e.g., for T = 25%, A = -log 0.25 = 0.602)
b. Use the graph paper on the back of this page (or a
spreadsheet) to plot absorbance vs. concentration.
Make a proper graph, with labeled axes, uniform
scales, etc. Use a ruler to draw the best-fit line..
C.
Use the graph to find the concentration of the un-
known sample. (Draw dotted lines on the graph
showing how you found your answer.)
CONCENTRATION PERCENT
(ppm)
TRANSMISSION
0
100.0
100
74.1
200
54.9
300
40.7
400
30.1
500
22.3
unknown
31.6
ABSORBANCE
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8
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Peak
Retention Time (minutes)
Area
1.201
136501204
acetone
2.011
1253056
toluene
ortho-product
para-product
3.201
8340750
3.350
14890645
a) What was the relative percent yield of the ortho-product?
b) What was the relative percent yield of the para-product?
c) What was the relative percent yield of the product?
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100
MASS SPECTRUM
80
60
40
20
0.0
10
20
30
40
50
60
m/z
NIST Chemistry WebBook (https://webbook.nist.gov/chemistry)
1. What is the M'?
2. What is the m/z of the base peak?
3. Does it contain chlorine? Explain your answer.
Rel. Intensity
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▪ True or False: In an untargeted analysis by GC-MS, we extract all acidic drugs
and all basic drugs using SPE with hydrophobic resin.
A)True
B)False
▪ In a comprehensive drug screen by GCMS, we set the detector to what mode and
why?
▪ A) Full scan; we don't know what analyted are in the sample, so we don't know what m/z to
focus the detector's time on.
▪ B) SIM; We have a good id
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0.200 M
0.00200 M
Test Tube No.
[FESCN2*1std (M)
Absorbance
Fe(NO3)3 (mL)
KSCN (mL)
17.98
1.97
0.815
5 (Standard)
Edit
Edit
Edit
NG 1
Info
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What is the retention factor (Rf) of the most polar and least polar spot present in the standard ink? (Use 2 significant figures)
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Please answer all and post below the answer the source from where you got the answer
thank you!
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A) Using the Data Graph below (with the equation for the best fit line), Camculate the concentration of a solution with an absorbance of 0.385.
B) Write the KC Expression for the Lab's Reaction.
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Please correct answer and don't use hand rating
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This is not a graded assignment, this is a question from a worksheet that I am teaching, and I wanted to check my answers please:On a graph of Absorbance and Concentration. There are three data points in addition to (0,0). The first point is 0.189 Absorbance for 2x10^-6MThe second is 0.378 Absorbance for 4x10^-6MThe third and last is 0.634 Absorbance for 6x10^-6M (slightly above the trendline)
a) If you recorded an absorbance of 0.525, calculate the corresponding molarity of the solution.
b) If the molar mass of the dye used was 791.41 g/mol, how many grams of dye were dissolved in 10.0 mLof the solution measured in part a?
c) What is the calculated absorbance for a solution with a concentration of 1.5x10-6 M?
d) Propose a logical reason for the error that occurred with the data point at 6.0 x10-6 M.
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Pre-Lab Assignment
Goals and objectives of the experiment should be
written in your laboratory notebook.
Pre-Lab Question 1. Make a list of the IR bands and
predict the absorption frequencies for the following
compounds.
a) 2-Chloropropionic acid
b) Methyl salicylate
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DATA REPORT FORM
Procedure A: Ink Analysis by Paper Chromatography
1. Attach the chromatography paper results here.
Name
2. Calculate the Rf value for each ink/marker sample. If there are more than one spot in the
sample, calculate and list Rf for all components.
Procedure B: Identification of Unknown Analgesic Drugs
1. Sketch the best results of the TLC plate here, including unknown sample and known
compounds.
11
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If the peak width w of both Peak 1 and Peak 2 fro eon 1g is 0.5
minutes, what is the resolution factor between the two peaks?
1.13
Selectivity is
Peak 1
(6.0 min,
Peak 2
w=0.5
(6.8 min,
w=0.5
25
min)
min)
20
15
Hold up peak
(1.8 min)
10
0.
0.
4.
8.
time (minutes)
2.
5.
Signal
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3) 3-Methylbutyramide
100
relative intensity
.8888
relative abundance
.88
CHINO
101
298 888
mass charge (m2)
CaH₁N
60
4) n-Butylamine
100
60
CHOCHOCHS
R98 8
mass charge (m/z)
MW=101.15
MW-73.13
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2.Dscribe The History of Forensic Chemistry?
3.Enumerate and discuss the functions of forensic Chemist.
4.Discussed the Purposed of the Golden rules in the practice of Forensic Chrmistry.
5.What is Toxicology.
6.Discussed the History of Toxicology.
7.Discussed Tbr collection of Sample for toxicology.
8.Discussed The importance of toxicology.
9.Discussed the Types of poison.
10. Discussed the entrance and elimination of poisons in Human body....
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Please help! I don't know how to even start
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ucumberlands.blackboard.com
Take Test: Quiz 3 - Spectroscopy
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Instructions
This test has a time limit of 50 minutes. You will be notified when time expires, and you may continue or submit.
Warnings appear when half the time, 5 minutes, 1 minute, and 30 seconds remain.
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Force Completion Once started, this test must be completed in one sitting. Do not leave the test before clicking Save and Submit.
Remaining Time: 22 minutes, 36 seconds.
Question Completion Status:
AMoving to another question will save this response.
Question 19
The amount of energy in infrared (IR) light corresponds to:
the amount of energy needed to increase certain molecular motions, such as bond vibrations, in organic molecules
the amount of energy needed to strip a molecule of one electron to generate a cation radical
the amount of energy needed to promote one electron from a bonding to an antibonding molecular…
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Related Questions
- Not exactly sure how to fill out the table?arrow_forwardHello, please answer the following attached Chemistry question correctly and fully based upon the attached table. Please answer the "Low and High absorbance" parts. Thank you.arrow_forwardThis is going with the first assignment I submitted. Fingerprintingarrow_forward
- Instruction: 1. Using a 25-mL glass stoppered buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/3 of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/3 of the content. Record the IR and FR of the second trial. 2. Using a 25-mL rubber stopperd buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/4of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/4 of the content. Record the IR and FR of the second trial.arrow_forwardInstruction: 1. Using a 25-mL glass stoppered buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/3 of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/3 of the content. Record the IR and FR of the second trial. 2. Using a 25-mL rubber stopperd buret filled with distilled water and with an initial reading (IR) of 0.00 mL, if about 1/4of the content is run down record the final reading (FR) for Trial 1. For trial 2 run down another 1/4 of the content. Record the IR and FR of the second trial. 3. Complete the table. 1. Buret with Glass Stopper TRIAL 1 TRIAL 2 Final reading Initial reading Volume Used 2. Buret with Rubber Stopper TRIAL 1 TRIAL 2 Final reading Initial reading Volume Usedarrow_forwardsub= 18 helparrow_forward
- PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.arrow_forwardUsing the lab instructions and data table with data, please help me complete part 1 and 2 of my lab worksheetarrow_forwardSt. John's University Department of Chemistry CHE2241L Date 427/2022 8:00AM Organic Lab Final Exam NAME 1) The attached picture shows the structure of aluminum oxide the column chromatography stationary phase that we used to separate fluorene from fluorenone. Iis this compound a) Polar 5) Nonpolar Explain why it is polar or nonpolar. It is because 2) Safety: Which of the following chemicals are extremely flammable: a) diethyl ether b) acetone c) methanol d) benzyl alcohol e) All of the above except d.arrow_forward
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Recommended textbooks for you
- Organic Chemistry: A Guided InquiryChemistryISBN:9780618974122Author:Andrei StraumanisPublisher:Cengage Learning
Organic Chemistry: A Guided Inquiry
Chemistry
ISBN:9780618974122
Author:Andrei Straumanis
Publisher:Cengage Learning