231L+Dyes+TEMPLATE 2023-04-18 20_33_05

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Iowa State University *

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MISC

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Chemistry

Date

Feb 20, 2024

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pdf

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13

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NEW TEMPLATE: 231L Synthetic Organic Dyes For this experiment you will by synthesizing an organic dye. You will use absorption spectroscopy, fluorescence characterization, and TLC to identify your dye. Pre-lab Questions Procedure and Safety 1. (10 pts) Write a bullet list outline for the procedure. 2. (5 pts) Write a brief summary of the key safety concerns specific for this experiment. Data and Observations (Only record for dye that you synthesized.) 1. (35 pts) Record what you are instructed to record in the procedure. In addition, while you are performing the synthesis of the dye that you chose, record any issues you had or what you could have done differently in order to get better results. There were no isssues in this lab, however I did get the wrong solution for the absorbance test. That issue was caught before getting the data though. REQUIRED DATA AND OBSERVATIONS FOR BOTH DYES (Record data for the dye that you synthesized. Obtain data for the other dye from a classmate.) 2. (5 pts) Insert a photo of dye 1 after rotovaping (to show color of compound).
3. (5 pts) Insert a photo of dye 2 after rotovaping (to show color of compound). (the other team did not take a picture of this) 4. (18 pts) Insert three photos showing absorption spectrum for dye 1, fluorescein, and 9- fluorenone. Indicate the wavelenth where maximum absorption occurred in each spectrum. Be sure to label each spectrum.
5. (18 pts) Insert three photos showing absorption spectrum for dye 2, eosin Y, and rhodamine B. Indicate the wavelenth where maximum absorption occurred in each spectrum. Be sure to label each spectrum.
6. (18 pts) Insert three photos showing fluorescence for dye 1, fluorescein, and 9-fluorenone. Be sure to label which photo is which compound.
7. (18 pts) Insert three photos showing fluorescence for dye 2, eosin Y, and rhodamine B. Be sure to label which photo is which compound. (other team didn’t provide 3 photos)
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