2. Formation of OAA in mitochondria. In the last reaction of the TCA cycle, malate is dehydrogenated to regenerate the OAA necessary for the entry of acetyl-coA into the cycle: L-Malate + NAD+ → OAA + NADH + H+ AGO' = 30.0 kJ/mol (a) Calculate the equilibrium konstant for this reaction at 25 C. b) Because AG assumes a standard pH of 7, the equilibrium constant calculated in (a) corresponds to [OAA][NADH]_ [L-malate][NAD*] K'eq = The measured concentration of L-malate in rat liver mitochondria is about 0.2 mM when the ratio [NAD*] / [NADH] is 10. Calculate the concentration of OAA at pH 7 in these mitochondria. (c) To appreciate the magnitude of the mitochondrial OAA concentration, calculate the number of OAA
2. Formation of OAA in mitochondria. In the last reaction of the TCA cycle, malate is dehydrogenated to regenerate the OAA necessary for the entry of acetyl-coA into the cycle: L-Malate + NAD+ → OAA + NADH + H+ AGO' = 30.0 kJ/mol (a) Calculate the equilibrium konstant for this reaction at 25 C. b) Because AG assumes a standard pH of 7, the equilibrium constant calculated in (a) corresponds to [OAA][NADH]_ [L-malate][NAD*] K'eq = The measured concentration of L-malate in rat liver mitochondria is about 0.2 mM when the ratio [NAD*] / [NADH] is 10. Calculate the concentration of OAA at pH 7 in these mitochondria. (c) To appreciate the magnitude of the mitochondrial OAA concentration, calculate the number of OAA
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter20: Electron Transport And Oxidative Phosphorylation
Section: Chapter Questions
Problem 11P
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![2. Formation of OAA in mitochondria. In the last reaction of the TCA cycle, malate is dehydrogenated to
regenerate the OAA necessary for the entry of acetyl-coA into the cycle:
L-Malate + NAD+ → OAA + NADH + H+
AGo = 30.0 kJ/mol
(a) Calculate the equilibrium ponstant for this reaction at 25 C.
b) Because AGo assumes a standard pH of 7, the equilibrium constant calculated in (a) corresponds to
K'eg
_JOAA][NADH]
[L-malate][NAD*]
The measured concentration of L-malate in rat liver mitochondria is about 0.2 mM when the ratio
[NAD*] / [NADH] is 10. Calculate the concentration of OAA at pH 7 in these mitochondria.
(c) To appreciate the magnitude of the mitochondrial OAA concentration, calculate the number of OAA
molecules in a single rat liver mitochondrion. Assume the mitochondrion is a sphere of diameter 2.0 µm.
(From problem 10, chapter 16)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7c9fffcb-a146-4185-bf71-b31903a19d20%2F1e9bc250-235e-437a-ae9e-df5252e696fb%2F6wtyigc_processed.png&w=3840&q=75)
Transcribed Image Text:2. Formation of OAA in mitochondria. In the last reaction of the TCA cycle, malate is dehydrogenated to
regenerate the OAA necessary for the entry of acetyl-coA into the cycle:
L-Malate + NAD+ → OAA + NADH + H+
AGo = 30.0 kJ/mol
(a) Calculate the equilibrium ponstant for this reaction at 25 C.
b) Because AGo assumes a standard pH of 7, the equilibrium constant calculated in (a) corresponds to
K'eg
_JOAA][NADH]
[L-malate][NAD*]
The measured concentration of L-malate in rat liver mitochondria is about 0.2 mM when the ratio
[NAD*] / [NADH] is 10. Calculate the concentration of OAA at pH 7 in these mitochondria.
(c) To appreciate the magnitude of the mitochondrial OAA concentration, calculate the number of OAA
molecules in a single rat liver mitochondrion. Assume the mitochondrion is a sphere of diameter 2.0 µm.
(From problem 10, chapter 16)
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