A 1L buffer solution needs to be prepared. The buffer will be composed of the following, 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol. The stock reagents for tris (124.14g/mol) and glycine (75.07g/mol) are stored as separate lyophilized powders. Glycerol is stored as a liquid at 20% (w/v). Approximately how many mL of glycerol should be measured for this buffer?
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A 1L buffer solution needs to be prepared. The buffer will be composed of the following, 200mM tris, 400mM glycine, and 0.5% (w/v) glycerol. The stock reagents for tris (124.14g/mol) and glycine (75.07g/mol) are stored as separate lyophilized powders. Glycerol is stored as a liquid at 20% (w/v). Approximately how many mL of glycerol should be measured for this buffer?
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- A 25 mL liquid bleach sample is diluted with water to 1000 mL in a bubble gel. 25 mL of the diluted sample is transferred to the flask with a pipette, excess KI is added to it, and thus OCl- ion turns into Cl- ion and I2 is formed. The released iodine is titrated with 0.09892 Na2S2O3 using the starch reagent, and 8.96 mL is required for the endpoint. Find the % NaOCl (w/v; w/v) in the bleach sample. (Na=23 g/mol, O= 16 g/mol, Cl = 35.44 g/mol) (Warning: solving the necessary reaction equations by writing is necessary to see the stoichiometric relationship)A 25 mL liquid bleach sample is diluted with water to 1000 mL in a bubble gel. 25 mL of the diluted sample is transferred to the flask with a pipette, excess KI is added to it, and thus OCl- ion turns into Cl- ion and I2 is formed. The released iodine is titrated with 0.09892 M Na2S2O3 using the starch reagent, and 8.96 mL is required for the endpoint. Find the % NaOCl (w/v; w/v) in the bleach sample. (Na=23 g/mol, O= 16 g/mol, Cl = 35.44 g/mol) (Warning: solving the necessary reaction equations by writing is necessary to see the stoichiometric relationship) A. 0.132% B. 0.264% C. 5.28% D. 10.56%A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3– was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL. C6H8O6 + I3– → C6H6O6 + 3I- + 2H+ I3– + 2S2O32- → S4O62- + 3I2 42.89 % 40.85 % 27.34 % 82.51 %
- A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3– was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL. C6H8O6 + I3– → C6H6O6 + 3I- + 2H+ I3– + 2S2O32- → S4O62- + 3I2 A. 82.51 % B. 42.89 % C. 27.34 % D. 40.85 %A solution containing orange rind extract was analyzed using gas chromatography to measure its limonene content. The parameters used to analyze the standards and samples are listed below. Calculate the concentration of limonene in the solution in ug/mLThe separation and purification processes given below and the method used against them are given. Which or which of these pairings are correct? I. Brewing of tea - ExtractionII. Purification of water impurities - ChromatographyIII. Separation of olive pomace from olive oil while producing olive oil-DecantationIV.Petroleum fractions obtaining - Ordinary distillationV. Obtaining essential oils and using them in perfume making - Water-steam distillation
- 6.0 mL of cyclohexanol, 8 mL of 85% phosphoric acid, and 10 drops of sulfuric acid are added to a 50-mL round-bottom flask with boiling chips, and the mixture is distilled into an ice-cooled receiver. The distillate is in the form of a colorless bi-phasic mixture. The cyclohexene upper layer is removed from the water layer, and dried over anhydrous calcium chloride. Isolated is cyclohexene as a clear and colorless foul-smelling liquid. Weighing data is given below. Gross mass: 51.24 g Tare mass: 48.04 g Product mass: 3.2g Question: Calculate the theoretical mass of the cyclohexene product, bearing in mind that the cyclohexanol starting material was measured by volume, not by mass (so you must use the density of cyclohexanol to convert milliliters to grams, before calculating the number of moles). Calculate the percent yield of cyclohexene obtained.2.1g of sample are dissolved in methylene chloride and the sample is extracted with aqueous solutions of acid and base. Three compounds are isolated - 0.8g of ethyl p-aminobenzoate, 0.6 g of acetanilide, and 0.5 g of benzoic acid. Calculate the % recovery for the procedure. (Round the answer to the nearest tens place.) Give just the number, not a % sign.)A 0.9000-g of KIO3 is dissolved in 500-mL distilled water. A 50.0-mL aliquot portion was run down into an Erlenmeyer flask and 2-g of KI and 2-mL of 6M HCl were added to it. Directly after, the solution was titrated with a 24.80-mL Na2S2O3 solution to a faint yellow solution. Starch TS was added to the resulting solution and the titration with the Na2S2O3 solution continued wherein an additional 1.33-mL was required to bring the blue color of the solution to disappear completely. MW: KIO3 = 214.0 g/mol ; Na2S2O3 = 158.1 g/mol Compute the N of the Na2S2O3 solution. None of the choices 0.09657 N 0.1075 N 0.1017 N
- In a solution containing 25ml (NH4)2S2O8 with a total volume = 100 ml =0.1 liters , the solution composition are shown below.Note; the solution require 25ml of 0.2M S2O8^-2.SOLUTION Kl KNO3 EDTA Na2S2O3 STARCH50ml 23ml 1 drop 1 ml 10 dropsExperiment; initial (S2O8-2) =0.05M ; INITIAL (l-)=0.10 Mml of S2O3^-2 added Time in minutes and seconds for color change Cumulative time in seconds Total moles of S2O8^-2 consumed1 1:43 2.0 x 10^-42 1:20 4.0 x 10^-43 1:13 6.0 x 10 ^-44 1.17 8.0 x 10 ^-45 1.24 10 x 10 ^-41a) Find the cumulative time in secondsb) for the runs ,plot mole of S2O8^2- REACTION vs. time in seconds2) Draw a straight line through ( origin ) the points and calculate the slope3) divide the value of the slope by the total volume of ( 0.1 L) to get the rate in units of M/s4) find the rate5 )calculate x and y and kWrite TRUE if the underlined word/phrase makes the statement correct. Otherwise, write the correct WORD/PHRASE that will make the statement true. d. Washing with hot solvent eliminates adhered mother liquor and avoids crystal dissolution. e. To extract a nonpolar organic compound using solvent extraction, the extracting solvent must be nonpolar, and the washing solvent must be also nonpolar. f. Addition of 6.0 M NaCl extracts saponins by converting them to their salt form. g. The stationary phase in the paper chromatography experiment is the filter paper. h. When the spots have low concentration, they will not be observed as visible spots in the chromatogram. i. An isomer in anti-staggered conformation has higher potential energy than the isomer in eclipsed conformation. j. The addition of excess HCl assures that the formation of tert-butyl chloride is favored. k. If the temperature during distillation of crude tert-butyl chloride reached 90°C, the distillate would contain tert-butyl…Calculate the weight percent of ascorbic acid in a tablet of Vitamin C using correct significant figures from the following data: A 90 mg sample of a crushed Vitamin C tablet was dissolved in 40 mL of H2SO4 and 20 mL of water. Two grams of KI and 20. mL of 0.0120 M KIO3 solution was added, and the mixture titrated to a starch endpoint. The titration required 12 mL of 0.0530 M thiosulfate solution. Weight = _____ %