Identification of spots on the TLC plate is done by all of the following EXCEPT O Spraying with reagents O Under microscope Ultraviolet O Ultraviolet adsorbent
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- how does the intermolecular force of attraction lead to stain removal?state referenceSeparation of Amino Acids by Thin Layer Chromatography Lab Questions. Please see photos and answer the questions relating to the images from #10. Thank you!Sequencing. Arrange the following procedures according to the CORRECT manner of preparing products. USE LETTERS IN SEQUENCING. 1. Turpentine Emulsion Preparation A. Triturate rapidly until the product is creamy while producing a crackling sound. B. Transfer to a graduate and made to volume with water. C. Solid substances like preservatives are dissolved in a suitable volume of water and are added to the primary solution. E. Add other formulative ingredients miscible with external phase AB. Triturate gum with oil in mortar until thoroughly mixed. AC. Add two parts of water all at once. 2. Methyl Salicylate Emulsion A. Add the remaining water to make final emulsion. B. Oil is first shaken thoroughly with the gum. C. Add the water all at once and shake vigorously. D. Add more water in small proportions with constant agitation in each addition. 3. Tragacanth Mucilage A. Mix water with glycerin and heat to boiling. B. Stir until uniform and strain if desired. C. Add water to make the…
- Enumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.[3] Instructions: Read each excerpt from a research paper and identify the dependent, independent, controlled variable (at least 1), experimental group/treatment, and control group. Antibacterial Assay The test solution of each extract was prepared by dissolving 0.1g of the plant extract separately. 1.0cm3 of dimethyl sulphoxide (DMSO) to get a concentration of 100mg/cm3. The antibacterial activity was performed by filter paper disc diffusion technique. Filter paper disc (Whatman No 1.6 mm diameter) were placed in glass Petri dish and sterilized in a hot air oven. Iwu et al 2018b, the media (10g nutrient Agar in 200cm3 distilled water, autoclaved at 115°C for 30 minutes) was cooled to 50°C. The sterile nutrient Agar media were poured into the sterile Petri dish and allowed to solidify. The bacteria were swabbed with a sterile wire loop. Each disc was impregnated with 0.2cm3 of plant extract. Standard antibiotic Ciprofloxacin was used as a control on a disc with DMSO 100 mg/cm3. The…1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asap