Most biochemists agree that the most accurate number of H+ needed to generate one ASP is blank. Therefore one NADH generated about blank ATP molecules?
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Most biochemists agree that the most accurate number of H+ needed to generate one ASP is blank. Therefore one NADH generated about blank ATP molecules?
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- Based on your knowledge of the structure of NAD+ and an assumption that coenzyme dissociation is the rate limiting step of the alcohol dehydrogenase mechanism, hypothesize why a N249W mutation at the coenzyme binding site would increase the rate of catalysis.Under standard conditions, NADH reoxidation by the electron-transport chain has a free-energy change equal to –220 kJ/mol. With 100% efficiency, how many ATP could be synthesized under standard conditions? What is the "actual" efficiency given these numbers?When 24 acetyl Co-A molecule(s) are respired through the Krebs Cycle and the electron transport chain to oxygen, how many ATP molecules will be produced?
- How do the P/O ratios for NADH differ in ATP synthases that contain 10 and 15 c subunits?Pyruvate oxidizes to form carbon dioxide and water and liberates energy at the rate of 1142.2 kJ/mol. If electron transport also occurs, approximately 12.5 ATP molecules areproduced. The free energy of hydrolysis for ATP is 230.5kJ/mol. What is the apparent efficiency of ATP production?Under standard conditions, is the oxidation of ubiquinol (Coenzyme Q) by O2 sufficiently exergonic to drive the synthesis of ATP? If yes, how many ATP can be synthesized assuming 100% efficiency?
- ATP Synthase is known to catalyze the synthesis of ATP with a ΔG°’ close to zero, and a Keq' close to. Why is the value of ΔG°’ different from the known value which is 30.5 kJ/mol (the energy for the reverse of ATP hydrolysis)? If the Keq' value is close to one, how is it ensured that the reaction is driven to the product side and more ATP is obtained?Assume that Compound A is an inhibitor of the electron transport chain's Complex II. On the other hand, Compound B is an uncoupler. What would be the effect of Compound A and Compound B on ATP production?It is estimated that each electron pair donated by NADH leads to the synthesis of approximately three ATP molecules, whereas each electron pair donated by FADH2 leads to the synthesis of approximately two ATP molecules. What is the underlying reason for the difference in yield for electrons donated by FADH2 versus NADH?
- Ethanol is oxidized in the liver to form acetate, which is then converted to acetyl-CoA. Determine how many molecules of ATP are produced from 1 mol of ethanol. (Note that 2 mol of NADH are produced when ethanol is oxidized to form acetate.)how many ATP synthesized by oxidation of NADH by O2? Compare it with the ATP synthesized by oxidation of NADH by nirate and H2S.Given what you know about the involvement of nicotinamide nucleotides inoxidative and reductive metabolic reactions, predict whether the followingintracellular concentration ratios should be 1, > 1, or < 1. Explain youranswers.(a) [NAD+] >[NADH](b) [NADP+] >[NADPH](c) Since NAD+ and NADP+ are essentially equivalent in their tendency to attract electrons, discuss how the two concentration ratios might bemaintained inside cells at greatly differing values.