tRNA charging -Amino acid - IRNA Ribosomal -Anticodon subunits UAC Large Start codon 5' AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' MRNA Small Stop codon Initiation UAC AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' 5' E PA Elongation Charged - TRNA AAAA, UC UCGCAA AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 5' 3' E PA Termination Termination Release factor UCG AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' 5' E PA Peptide release Completed polypeptide AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' Conclusion: Through the process of translation, amino acids are linked in the order specified by the MRNA. 15.21 Translation requires tRNA charging, initiation, elongation, and termination.

tRNA charging -Amino acid - IRNA Ribosomal -Anticodon subunits UAC Large Start codon 5' AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' MRNA Small Stop codon Initiation UAC AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' 5' E PA Elongation Charged - TRNA AAAA, UC UCGCAA AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 5' 3' E PA Termination Termination Release factor UCG AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' 5' E PA Peptide release Completed polypeptide AUGCCCACGACUGCGAGCGUUCCGCUAAGGUAG 3' Conclusion: Through the process of translation, amino acids are linked in the order specified by the MRNA. 15.21 Translation requires tRNA charging, initiation, elongation, and termination.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter30: Protein Synthesis

Section: Chapter Questions

Problem 6P

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mRNA sequence of A gene If we have the following mutations, find the type of the mutation (silent or missense or nonsense?) 17CàU 36GàA 49GàU 115AàC 5’ AAACUGUGACUGAACCUCAAACCCCAAACCAGCCCGAGGAGAACCACAUUCUCCCAGGGA CCCAGGGCGGGCCGUGACCCCUGCGGCGGAGAAGCCUUGGAUAUUUCCACUUCAGAAGCC UACUGGGGAAGGCUGAGGGGUCCCAGCUCCCCACGCUGGCUGCUGUGCAGAUGCUGGACG ACAGAGCCAGGAGGGAGGCCGCCAAGAAGGAGAAGGUAGAGCAGAUCCUGGCAGAGUUCCAGC UGCAGGAGGAGGACCUGAAGAAGGUGAUGAGACGGAUGCAGAAGGAGAUGGACCGCGGCCUGA GGUAGAAGCCGCUGGGGCUUGGGGCU-3’
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From this overall anticodon sequence in tRNA, 3'-CAUCGGAAUAGAUCGCUAGUGGCAGGCAUAAUGAUCACCGGUCUGAGAAAAGUGGUACAUAUCAAC-5' What is the amino acid sequence that will be coded for using ONE-letter amino acid code starting from N-terminus to C-terminus and using THREE-letter amino acid code starting from N-terminus to C-terminus
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