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You add one mL of bacterial culture to 9 mL of water.  From this tube, you take 1 mL and add it to a new tube containing 9 mL of water.  What is the resulting dilution factor?

Question

You add one mL of bacterial culture to 9 mL of water.  From this tube, you take 1 mL and add it to a new tube containing 9 mL of water.  What is the resulting dilution factor?

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Step 1

A serial dilution is the stepwise dilution of a substance in solution. Usually, the dilution factor at each step is constant, resulting in geometric progression of the concentration in a logarithmic fashion.

Step 2

When 1mL of bacterial culture is added to 9 mL of water, a 10mL of the solution is prepared. This solution has 1mL of the bacterial culture per 10mL, producing a 10-fold dilution. When ...

Dilution factor (DF) = Vi / Vf
Where, Vi initial volume of the aliquot
Vf
final volume obtained by adding the aliquot to the diluent
DF 1mL (1 9) mL
= 1/ 10
Therefore, 1:10 dilution
When 1ml of this solution was added to 9 mL of water again, then the dilution
factor after two dilutions would be:
DF (1 10) X (1 /10)
=1/100
Therefore, the resulting dilution factor would be 1:100
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Dilution factor (DF) = Vi / Vf Where, Vi initial volume of the aliquot Vf final volume obtained by adding the aliquot to the diluent DF 1mL (1 9) mL = 1/ 10 Therefore, 1:10 dilution When 1ml of this solution was added to 9 mL of water again, then the dilution factor after two dilutions would be: DF (1 10) X (1 /10) =1/100 Therefore, the resulting dilution factor would be 1:100

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