Question
Asked Sep 30, 2019
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You were instructed to add 1.0 ml out of 5.0 mL of an undiluted sample to 99 mL of sterile diluted instead you add the entire 5.0 ml to 99 ml. 

What was your intended dilation Factor

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Expert Answer

Step 1

According to this question

The volume of sterile dilute= 99ml(milliliters)

The volume of undiluted sample to be added=1ml

The intended dilute factor was 1:100

Step 2

Dilution are always the amount of sample added over the total volume:

 

volumeofsample
Therefore, the dilution factor = totalvolumeofsolution
1
99+1
1
100
=0.01
II
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volumeofsample Therefore, the dilution factor = totalvolumeofsolution 1 99+1 1 100 =0.01 II

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Step 3

In this case, all of the undiluted sample was ...

volumeofsample
Therefore, the dilution factor = totalvolumeofsolution
5
99+5
5
104
1
20.8
=0.048
help_outline

Image Transcriptionclose

volumeofsample Therefore, the dilution factor = totalvolumeofsolution 5 99+5 5 104 1 20.8 =0.048

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