GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 10, Problem 12P
Summary Introduction
To analyze:
Why the positions of
Introduction:
Blotting is the technique routinely used for the detection of
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Describe the steps, starting from an endonuclease digested DNA sample, to complete a Southern blot experiment.
What difference would a Northern blot have?
A Western blot is seen in the picture above. Sodium dodecyl sulfate gel polyacrylamide gel electrophoresis was used to isolate the proteins (SDS-PAGE).1. Since gel electrophoresis has isolated individual proteins, what kind of molecule is used to detect them? 2. On the right side of this Western blot, three molecules are identified. One of the bands corresponds to the smallest molecule?
Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands will be 4 conditions x 3 proteins (phospho-S6, phospho-AMPK, tubulin). Please describe the relative density of each band compared to the control (for example, how dense will phospho-S6 be in each of the three experimental conditions compared to the control condition?). For each band, provide a well-reasoned rationale for your anticipated result. Give the reason why cell signaling would produce each result in each condition.
Chapter 10 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 10 - Define the following terms as described in this...Ch. 10 - 2. Using sickle cell disease as an example,...Ch. 10 -
3. Compare and contrast the contributions of...Ch. 10 - Why do differences in protein electrophoretic...Ch. 10 - Prob. 5PCh. 10 - Prob. 6PCh. 10 - Prob. 7PCh. 10 - 8. Wildtype βglobin protein is composed of amino...Ch. 10 - Prob. 9PCh. 10 - Prob. 10P
Ch. 10 - 11. How is an autoradiograph produced from a...Ch. 10 - Prob. 12PCh. 10 - Prob. 13PCh. 10 - Prob. 14PCh. 10 - The family represented in the pedigree and...Ch. 10 - Suppose the mating couple (I-1 and I-2) shown in...Ch. 10 - What are restriction endonucleases, and why are...Ch. 10 - 18. Following restriction digestion, DNA fragments...Ch. 10 - 19. The doublestranded DNA sequence below is part...Ch. 10 - 20. Restriction enzymes recognize specific...Ch. 10 - Prob. 21PCh. 10 - Prob. 22PCh. 10 - Prob. 23PCh. 10 - Prob. 24PCh. 10 - 25. A second strain of dwarf plants has a...Ch. 10 - During gel electrophoresis of linear DNA...Ch. 10 - Prob. 27PCh. 10 - 28. In molecular biology, restriction...Ch. 10 - A complete plant gene containing four introns and...Ch. 10 - Prob. 30PCh. 10 - The map below illustrates three alleles in a...Ch. 10 - Prob. 32PCh. 10 - 33. Northern blot analysis is performed on mRNA...
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- Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over time.arrow_forwardCoomassie dye is the equivalent of what component used in analysis of DNA by agarose gel electrophoresis? How do you expect a Coomassie-stained gel to look compared to the result you might expect once you have completed your Western blot?arrow_forwardFor your Southern Blot, every band showed a reaction with the probe.Describe what happened and how you could correct for it.arrow_forward
- In the Western blot shown here, proteins were isolated from redblood cells and muscle cells from two different individuals. Oneindividual was unaffected, and the other suffered from a diseaseknown as thalassemia, which involves a defect in hemoglobin. Theblot was exposed to an antibody that recognizes β globin, whichis one of the polypeptides that constitute hemoglobin. Equal totalamounts of cellular proteins were added to each lane. Explain these results.arrow_forwardSouthern and Northern blotting are powerful molecular biological tools based on hybridization of nucleic acids. How are these techniques the same? How do they differ? Give some specific applications for each blotting technique.arrow_forwardIn Colony Polymerase Chain Rxn (PCR), what primers are necessary to identify that the transformants contain the gene of interest with the correct orientation?arrow_forward
- Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch Repair (2) Nucleotide-Excision Repairarrow_forwardWhich of the following pieces of information about our recombinant DHFR protein do you predict to obtain from Western Blot analysis? A) Protein size B) Protein sequence C) Protein functionarrow_forwardDiagram and explain how APEX probes can be used to determine that an individual is CC (homozygous) for a specific G/C SNV. Recall that the genotype of an SNV is identified from the strand shown in NCBI. What color fluorescence will be observed? Also, explain why a left apex probe cannot be used for this SNV. The SNV sequence, on the strand shown in NCBI, and a few nucleotides adjacent to the SNV are below: 5'-------TGT(G/C)CAG------3'arrow_forward
- How does CRISPR work? (Not, "What is CRISPR?" although that is important to know, but how does it work?)arrow_forwardYou run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker band. What is the most appropriate interpretation of this? (a) sample A has more Ras DNA, (b) sample B has more Ras DNA, (c) sample A has more Ras mRNA, (d) sample B has more Ras mRNA, (e) sample A has more Ras protein, (f) sample B has more Ras protein. I understand that those molecules have higher molecular weight should give darker band. As mRNA has higher molecular weight than that of DNA and protein, I wish to pick (d) "sample B has more Ras mRNA" as the best answer. I am not sure if I am right. Please the expert advise.arrow_forwardLooking at the sequence of gene X, you hypothesize that there is an intrinsic terminator in the 3' end of gene X. To test your hypothesis, you delete the putative intrinsic terminator (AIT). Which of the following northern blots for gene X would you expect if your hypothesis is correct (that the IT sequence is an intrinsic terminator). wild-type A Blot B O Blot D Blot E O Blot A AIT O Blot C AIT wild-type B wild-type с ΔΙΤ wild-type D ΔΙΤ wild-type E ΔΙΤarrow_forward
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