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Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 12, Problem 9P
Two wild-type fragments of human genomic DNA from the long arms of two nonhomologous acrocentric chromosomes are shown in the accompanying diagram. On both fragments, the centromere-to-telomere direction is left-to-right. The red lines numbered 1, 2, and 3 indicate the positions of breakpoints that could be caused by ionizing radiation. Certain combinations of these breaks could produce chromosomal rearrangements when the broken pieces are stitched back together by DNA repair systems.
You will want to diagnose the presence of the rearrangements by using PCR. In every case that follows, one of the primers you will use for PCR is primer A, whose sequence is:
5′ TCGATTCCGGAAAGCT 3′
a. | Which two of the breakpoints could yield a deletion? |
b. | Write the sequence and polarity of a 16-base primer that, in conjunction with primer A, will allow you to diagnose the presence of a deletion in a patient’s genomic DNA. The evidence should be positive, not negative. (That is, you will see a PCR product whose presence and/or size is specific to the deletion; the absence of a band is not informative.) |
c. | Which two of the breakpoints could yield an inversion? |
d. | Write the sequence and polarity of a 16-base primer that, in conjunction with primer A, will allow you to diagnose the presence of an inversion in a patient’s genomic DNA. Your answer should show the primer that would yield the longest possible diagnostic PCR product. Your primer cannot cross any of the red lines. |
e. | Which two of the breakpoints could yield a reciprocal translocation? (Two possible answers exist; you need only write one.) |
f. | Write the sequence and polarity of a 16-base primer that, in conjunction with primer A, will allow you to diagnose by PCR the presence of a reciprocal translocation in a patient’s genomic DNA. This reciprocal translocation can be stably inherited through meiosis without its loss or any chromosomal breakage. Again, the evidence should be positive, not negative. |
g. | Is the translocation you described in parts (e) and (f) Robertsonian? Answer yes or no, and explain.![]() |
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Chapter 12 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 12 - For each of the terms in the left column, choose...Ch. 12 - Prob. 2PCh. 12 - For each of the following types of chromosomal...Ch. 12 - For the following types of chromosomal...Ch. 12 - One of the X chromosomes in a particular...Ch. 12 - Prob. 6PCh. 12 - Prob. 7PCh. 12 - Prob. 8PCh. 12 - Two wild-type fragments of human genomic DNA from...Ch. 12 - Indicate which of the four major classes of...
Ch. 12 - The recessive, X-linked z1 mutation of the...Ch. 12 - Genes a and b are 21 m.u. apart when mapped in...Ch. 12 - In the following group of figures, the pink lines...Ch. 12 - Three strains of Drosophila Bravo, X-ray, and...Ch. 12 - Prob. 15PCh. 12 - Suppose a haploid yeast strain carrying two...Ch. 12 - In the mating between two haploid yeast strains...Ch. 12 - During ascus formation in Neurospora, any...Ch. 12 - In the following figure, black and pink lines...Ch. 12 - In Drosophila, the gene for cinnabar eye color is...Ch. 12 - Semisterility in corn, as seen by unfilled ears...Ch. 12 - Prob. 22PCh. 12 - Prob. 23PCh. 12 - Prob. 24PCh. 12 - Duchenne muscular dystrophy DMD is caused by a...Ch. 12 - Explain how transposable elements can cause the...Ch. 12 - The Drosophila genome normally harbors about 40 P...Ch. 12 - Prob. 28PCh. 12 - Fred and Mary have a child named Bob. The genomic...Ch. 12 - Uniparental disomy is a rare phenomenon in which...Ch. 12 - Among adults with Turner syndrome, it has been...Ch. 12 - In Neurospora, his2 mutants require the amino acid...Ch. 12 - Human geneticists interested in the effects of...Ch. 12 - The incidence of Down syndrome will be very high...Ch. 12 - The Drosophila chromosome 4 is extremely small;...Ch. 12 - Down syndrome is usually caused by having a...Ch. 12 - Common red clover, Trifolium pratense, is a...Ch. 12 - The numbers of chromosomes in the somatic cells of...Ch. 12 - Prob. 39PCh. 12 - Somatic cells in organisms of a particular diploid...Ch. 12 - Prob. 41PCh. 12 - Prob. 42PCh. 12 - Chromosomes normally associate during meiosis I as...Ch. 12 - Using whole-genome sequencing, how could you...Ch. 12 - Prob. 45PCh. 12 - Prob. 46PCh. 12 - Seedless watermelons that you find in the...Ch. 12 - Prob. 48PCh. 12 - What characteristic property of translocations...Ch. 12 - In examining the genome of the rice Oryza sativa...Ch. 12 - Prob. 51PCh. 12 - Prob. 52PCh. 12 - The accompanying figure shows idiograms of human...
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