The technique to modify standard quantitative PCR to quantify the level of a specific mRNA. Introduction: Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Question

Want to see the full answer?

Answer 1

Question

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Why are entire genomes not used for DNA profiling?

Is it necessary to cut the isolated genomic DNA of an organism with restriction enzymes for analyzing a particular gene sequence? Discuss.

What determines the size (length) of the primary PCR product? What might a successful gel check of a PCR reaction look like?

Answer 2

Question

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Answer 6

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Answer 7

Chapter 12.2, Problem 3TQ

Summary Introduction

To review:

The technique to modify standard quantitative PCR to quantify the level of a specific mRNA.

Introduction:

Quantitative PCR (polymerase chain reaction) or real-time PCR is amodification of the PCR technique. This technique uses fluorescence to monitor the progress through the PCR with time. The products are analyzed during progression. Quantitative PCR can be used to quantify the DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) molecules in a sample. The time at which the fluorescence increases reflects the amount of nucleic acid present in the original sample.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 12.1 - Prob. 1TQ Ch. 12.1 - Prob. 2TQ Ch. 12.1 - Prob. 3TQ Ch. 12.1 - Prob. 4TQ Ch. 12.2 - Prob. 1TQ Ch. 12.2 - Prob. 2TQ Ch. 12.2 - Prob. 3TQ Ch. 12.3 - Prob. 1TQ Ch. 12.4 - Prob. 1TQ Ch. 12.4 - Prob. 2TQ

Solution Summary: The author describes Quantitative PCR (polymerase chain reaction) and RT-qPCR, which is used to quantify the level of a specific mRNA.

Concept explainers

Videos

Want to see the full answer?

Chapter 12 Solutions