Concept explainers
A researcher has a Trp auxotrophic strain of E. coli with a mutation in a single gene. To identify that mutant
gene, she uses a genomic library made from a wild-type version of that same strain to find plasmids that rescue the mutant
of the plasmids contain gene X, while the other four contain gene Y. Our scientist has encountered a
phenomenon called multicopy suppression, related to the fact that plasmids are usually present in several copies per bacterium. Because the genes in the plasmids are present in more than their usual single copy in the
bacterial chromosome, more than the usual amount of Protein X or Protein Y is being produced from the plasmids. Sometimes, overexpression of one protein can rescue the mutant phenotype caused by loss of a
different protein. Suggest at least two ways that our scientist could determine which of the two genes, gene X or gene Y, actually corresponds to the mutant gene causing the Trp phenotype.
Want to see the full answer?
Check out a sample textbook solutionChapter 14 Solutions
Genetics: From Genes to Genomes
- Construct a restriction map for Plasmid X to show the recognition sites for two commonly used restriction enzymes: EcoRI and BamHI. Plasmid X undigested Plasmid X digested with EcoRI Plasmid X digested with BamHI Plasmid X digested with EcoRI and BamHI 2200 bp 1000 bp 1200 bp 400 bp 1800 bp 200 bp 400 bp 600 bp 1000 bparrow_forwardYou are attempting to clone a 3 kb gene from the bacterial sp Microbacterium foliarum into the EcoRI site of the 6.0 kb plasmid shown. If you restrict the plasmid with EcoR1, how many bands will you obtain on agarose gel electrophoresis? Compare it with a plasmid in which no gene has been inserted. Draw a representative agarose gel showing the bands, and the direction of current flow as well point out the positive and negative electrodesarrow_forwardHow do you know if you’re purified plasmids contain your mutation? Why or why not?arrow_forward
- What is an engineered plasmid used for? What are the important features of\ engineered plasmids?arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forwardWhat is true regarding the following cross between bacterial strains: F’ x F-? As the donor, F' will donate only the F plasmid to F- As the donor, F- will donate the F plasmid only to F' As the donor, F- will donate the F plasmid and a portion of the bacterial chromosome to F' As the donor, F' will donate the F plasmid and a portion of the bacterial chromosome to F-arrow_forward
- Why in healthcare infections, genes conferring antibiotic resistance are mostly resided on R plasmids?arrow_forwardHow is an R plasmid similar to an F plasmid?arrow_forwardWhat is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic transformation should have?arrow_forward
- If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a plasmid, and transform it into E. coli, will I be able use the transformed E. coli to make the corresponding protein? Explain why, or why not? If you decide to do this, what would your cloning strategy be?arrow_forwardIf you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert? c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?arrow_forwardCan you separate a plasmid from a non plasmid nucleic acid? How?arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education