Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
11th Edition
ISBN: 9780133910605
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 2FIB
_________is the process whereby bacteria pick up DNA from their environment. This DNA may be part of a chromosome or It may be tiny circles of DNA called _______.
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Plasmids are __________ DNA molecules that replicateautonomously and can be transferred between cells; theyoften contain genes for drug resistance or pathogenicity,or for specialized metabolic functions
A genomic DNA library is made by using _____ to cut the target genome into small fragments, which are then amplified by PCR and ligated into plasmids.
________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host.
Group of answer choices
1. Restriction enzymes; ethidium bromide
2 DNA polymerases; ligase
3 Restriction enzymes; ligase
4 Ligases; restriction enzymes
Chapter 14 Solutions
Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Guilty or Innocent? When biological evidence was...Ch. 14.3 - For any single person, a given STR always has...Ch. 14.3 - Prob. 2CSCCh. 14.3 - There are many other applications in which DNA...Ch. 14.3 - Prob. 1CYLCh. 14.3 - Prob. 2CYLCh. 14.3 - Prob. 3CYL
Ch. 14.4 - Restriction enzymes are isolated from bacteria....Ch. 14.4 - explain how genes are inserted into a plasmid, and...Ch. 14.4 - Prob. 2CYLCh. 14.5 - Prob. 1HYEWCh. 14.5 - describe the advantages of genetically modified...Ch. 14.5 - list some examples of how GM animals might be...Ch. 14.5 - Prob. 3CYLCh. 14.6 - Prob. 1CYLCh. 14.6 - explain how knowledge of the genomes of humans and...Ch. 14.7 - Prob. 1TCCh. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - Genetic engineering is used both in food crops and...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - envision circumstances in which it would be...Ch. 14.8 - Prob. 1CSRCh. 14.8 - Prob. 2CTCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- Conjugation is the uptake of DNA from the environment. true or falsearrow_forwardMultiple antibiotic resistance genes are contained on a bacterial plasmid in a __________. circular region of RNA a series of interlocking proteins the region that also contains genes for making the pilius needed to transfer the plasmid (Incorrect, already attempted) the resistance cassette Thank you!arrow_forwardWhich statement about plasmid is FALSE: Plasmid is nonessential DNA in the bacteria Plasmids replicate independent of the genome replication. Their size can vary from a few kilobases to a few hundred kilobases. They are all circular.arrow_forward
- Black men are more likely to get prostate cancer than other men. You are working on a prostate cancer project. You want to isolate cancer cells from a diseased prostate and culture the cells. Akilah, your lab partner uses a procedure to isolate the prostate cancer cells. You decide you want to culture the cells for various types of biological analysis. Write up the procedure used to culture the prostate cancer cells You froze some of the prostate cancer cells for future experiments. How did you freeze the cells?Now you and your team decided to analyze the genetic material. Your lab mate isolated the DNA from the prostate cancer cells. You isolated the RNA from the prostate cancer cells. Write up the procedure you used to isolate the RNA Your lab mate does Northern blotting to visualize the RNA expression. You are interested in two genes that are overexpressed in prostate cancer cells. Write up or illustrate the procedure you used to run the Northern blot in your own words.Alex,…arrow_forwardA ____________ is required to transfer DNA sequences from one organism to another. reporter gene vector genetic probe restriction endonuclease genomic libraryarrow_forwardStatement 1: Plasmids are small, linear pieces of DNA that can be found in bacteria. Statement 2: Plasmid acts as a cloning vectors, a DNA molecule that can carry foreign DNA into a host cell and be replicated there. Both statements are true. Both statements are false. O statement 1 is true. Statement 2 is false. Statement 1 is false. Statement 2 is true.arrow_forward
- The most usefulplasmids contain a _______, which is a short, syntheticDNA sequence that contains a number of different restriction enzyme restriction sitesarrow_forwardAs shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.arrow_forwardThe presence of a specific plasmid in a bacterial cell may be determined if the plasmid __________. contains pieces of a viral genome contains a selectable marker is a vector is exceptionally large All of the above are correctarrow_forward
- Which of the following statements is true about DNA molecule migration through a gel? Group of answer choices Size does not affect the rate of migration. Small fragment migration is slower Large fragment migration is farther. Small fragment migration is fasterarrow_forwardBecause strawberries have 8 sets of chromosomes, they yield a lot more DNA compared to that of cheek cells. But, DNA looks the same whether it is extracted from plants or animals. Please describe the appearance of the DNA.arrow_forwardNew alleles in bacteria can arise by All of the answers are correct. conjugation transduction transformation mutationarrow_forward
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