Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
11th Edition
ISBN: 9780133910605
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 4MC
A restriction enzyme
- a. cuts DNA at a specific
nucleotide sequence. - b. cuts DNA at a random nucleotide sequence.
- c. splices pieces of DNA together at a specific nucleotide sequence.
- d. splices pieces of DNA together without regard to the nucleotide sequence.
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Students have asked these similar questions
Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand. Sticky ends are useful in making recombinant DNA because
Select one:
a.  Sticky ends contain the exact same nucleotides that allows fragments to splice together.
b.  Sticky ends contain nucleotides with complementary bases that allows fragments to splice together.
c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds.
d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.
DNA polymerases make errors in matching as DNA is synthesized. These errors can be detected and repaired by:
Â
A.
Ligase
Â
B.
DNA polymerase
Â
C.
Primase
Â
D.
Helicase
Which of the following best describes the process of DNA seqencing.
a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser.
b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragmentsÂ
c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR.
d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser
e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laser
Chapter 14 Solutions
Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Guilty or Innocent? When biological evidence was...Ch. 14.3 - For any single person, a given STR always has...Ch. 14.3 - Prob. 2CSCCh. 14.3 - There are many other applications in which DNA...Ch. 14.3 - Prob. 1CYLCh. 14.3 - Prob. 2CYLCh. 14.3 - Prob. 3CYL
Ch. 14.4 - Restriction enzymes are isolated from bacteria....Ch. 14.4 - explain how genes are inserted into a plasmid, and...Ch. 14.4 - Prob. 2CYLCh. 14.5 - Prob. 1HYEWCh. 14.5 - describe the advantages of genetically modified...Ch. 14.5 - list some examples of how GM animals might be...Ch. 14.5 - Prob. 3CYLCh. 14.6 - Prob. 1CYLCh. 14.6 - explain how knowledge of the genomes of humans and...Ch. 14.7 - Prob. 1TCCh. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - Genetic engineering is used both in food crops and...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - envision circumstances in which it would be...Ch. 14.8 - Prob. 1CSRCh. 14.8 - Prob. 2CTCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- Certain restriction endonucleases produce cohesive (sticky) ends. Â This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. Â make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. Â d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forwardHow would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerasearrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forward
- You are presented with the following DNA molecule: 5’ A T G C G A T T A T A A 3’ 3’ T A C G C T A A T A T T 5’ A. Write the template DNA strand B. Transcribe the template DNA strand into mRNA C. Translate the mRNA into an amino acid sequencearrow_forwardDNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardUse the following information to answer the next question In the process of genetic engineering, recombinant DNA is produced by combining genetic material from two different sources. For this technique, genetic engineers are especially interested in a specific group of enzymes called restriction endonuclease. These enzymes are preferred because they join the DNA fragments at 3' ends join the DNA fragments at 5' ends cut DNA at specific sites within the DNA cut DNA from their 5' or 3' endsarrow_forward
- Enzyme function is critically important for the proper replication of DNA. Predict the consequence of a loss of function for each of the following enzymes. a. DNA gyrase b. DNA polymerase III c. DNA ligase d. DNA polymerase Iarrow_forwardWhich of the following is NOT true of cutting DNA in biotechnology? O A. A molecular biologist can cut DNA using restriction enzymes. O B. DNA can be cut at specific base sequences called recognition sequences. OC. When DNA is cut in such a way that some of the bases are no longer paired with their complements, the unpaired ends of that DNA fragment are called sticky ends. If the piece of DNA shown below is cut with an enzyme that cuts at "GGCC", 3 fragments would result. DNA: GTATGACTGAATTCCCAGGCCATCTCCGGAarrow_forwardThe short Okazaki fragments are Select one: a. spliced together by DNA ligase b. glued together by RNA primers c. fused together by DNA polymerase d. formed into the lagging strand without splicingarrow_forward
- Which of the following enzymes is responsible for "exposing" or "unwinding" the DNA template (taking it out of its double helix) for DNA Replication?  a. Topoisomerase  b. Telomerase  c. DNA Ligase  d. Primase  e. Helicasearrow_forwardSome bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: Select one: O a. DNA polymerases O b. DNA ligases c. restriction endonucleases O d. restriction ligasesarrow_forwardA cloned fragment of DNA was sequenced by using thedideoxy chain-termination method. A part of the autoradiogram of the sequencing gel is represented here.ddA ddG ddT ddCa. Deduce the nucleotide sequence of the DNAnucleotide chain synthesized from the primer. Label the5′ and 3′ ends.b. Deduce the nucleotide sequence of the DNAnucleotide chain used as the template strand. Label the5′ and 3′ ends.c. Write out the nucleotide sequence of the DNA doublehelix (label the 5′ and 3′ ends)arrow_forward
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