Chapter 14, Problem 5MC

Summary Introduction

Introduction:

$\text{DNA}$ Cloning is a method of introducing a foreign $\text{DNA}$ inside the genome of a foreign organism leading to the formation of genetically modified organisms. The restriction enzymes cut specific palindromic sequences of $\text{DNA}$ which are cloned inside the multiple cloning sites of a plasmid. This plasmid is then inserted inside a bacterial or a mammalian cell leading to the expression of foreign genes. The gene is amplified by the method of polymerase chain reaction leading to the production of multiple copies of $\text{DNA}$.