Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
11th Edition
ISBN: 9780133910605
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14, Problem 3MC
Summary Introduction
Introduction:
Polymerase chain reaction involves the amplification of
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Which of the following is not a commonly used method of modifying the DNA of an organism?a. crossbreeding two plants of the same speciesb. crossbreeding two plants of different speciesc. the polymerase chain reactiond. genetic engineering
When constructing a recombinant DNA molecule, a marker gene is used to:
a.
give the organism a new trait, such as insect resistance
b.
Identify whether the transformed organism contains the recombinant DNA
c.
replicate (copy) the gene of interest
d.
Introduce the recombinant DNA into an organism
e.
cut short sequences of DNA
Which is the BEST technique to use to answer this question?
You wish to determine whether a point mutation is present in the sunscreen gene.
a. Westem blot.
b. Northern blot .
c. Sanger DNA sequencing
d. In situ hybridization.
Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo.
a. Immunofluorescencez
b. Western blot .
c. RT-PCR.
d. Northern blot.
e. in situ hybridization.
Chapter 14 Solutions
Biology: Life on Earth with Physiology Plus Mastering Biology with Pearson eText -- Access Card Package (11th Edition)
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Guilty or Innocent? When biological evidence was...Ch. 14.3 - For any single person, a given STR always has...Ch. 14.3 - Prob. 2CSCCh. 14.3 - There are many other applications in which DNA...Ch. 14.3 - Prob. 1CYLCh. 14.3 - Prob. 2CYLCh. 14.3 - Prob. 3CYL
Ch. 14.4 - Restriction enzymes are isolated from bacteria....Ch. 14.4 - explain how genes are inserted into a plasmid, and...Ch. 14.4 - Prob. 2CYLCh. 14.5 - Prob. 1HYEWCh. 14.5 - describe the advantages of genetically modified...Ch. 14.5 - list some examples of how GM animals might be...Ch. 14.5 - Prob. 3CYLCh. 14.6 - Prob. 1CYLCh. 14.6 - explain how knowledge of the genomes of humans and...Ch. 14.7 - Prob. 1TCCh. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - Genetic engineering is used both in food crops and...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - envision circumstances in which it would be...Ch. 14.8 - Prob. 1CSRCh. 14.8 - Prob. 2CTCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- What is a genetically modified organism (GMO)? a. a plant with certain genes removed b. an organism with an artificially altered genome c. a hybrid organism d. any agricultural organism produced by breeding or biotechnologyarrow_forwardGenetic engineering is the direct modification of an organisms’ DNA using biotechnology. What are the different techniques in genetic engineering? a. Inoculate, Gel electrophoresis, Budding and Cloning b. Grafting, Gene splicing, Budding and Inbreeding c. Cloning, Gene splicing, Gel electrophoresis and Hybridization d. Gene splicing, Grafting, Hybridization and Cloningarrow_forwardGenetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresis b. polymerase chain reactionc. transgenic bacteriad. DNA fingerprintingarrow_forward
- Which is the proper order of the following steps in a genecloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4 c. 2, 4, 1, 3b. 2, 3, 1, 4 d. 1, 2, 4, 3arrow_forwardFor each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.arrow_forwardWhich of the following is not essential to carry out the polymerasechain reaction?a. primers b. DNA polymerase c. gel electrophoresis d. high temperaturearrow_forward
- The picture below depicts an aberration in the process of genetic coding. Which of the following terms best describes this occasional occurrence in genetic material? see photo attached a. Chromosomal aberration b. Mutation c. CRISPR d. ZNFarrow_forwardWhat is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- Imagine you wanted to preserve the dwindling populations of giant pandas by developing breeding programs for captive pandas around the world. To preserve the maximum genetic diversity, you want to mate the individuals that are least related to each other. Which of the following methods of analysis would be most helpful in determining which of the captive pandas were related? a. PCR b. STR analysis c. DNA microinjection d. DNA sequencingarrow_forwardPCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardWhich of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.arrow_forward
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