Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Chapter 14, Problem 5RQ
The experiments by Hershey and Chase helped confirm that DNA was the hereditary material on the basis of the finding that:
- radioactive phage were found in the pellet
- radioactive cells were found in the supernatant
- radioactive sulfur was found inside the cell
- radioactive phosphorus was found in the cell
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The experiments by Hershey and Chase helped confirm that DNA was the hereditary material on the basis of the finding that: a. radioactive phage were found in the pellet b. radioactive cells were found in the supernatant c. radioactive sulfur was found inside the cell d. radioactive phosphorus was found in the cell
if You have been transported to a DNA Lab by a mad scientist. The scientist hands you a gel electrophoresis rig and he tells you to put the anode (+) and the cathode (-) on the rig correctly or... else. You have never run an electrophoresis rig in your life, nor do you know exactly what it does. However, you should be able to save your life knowing what you know about DNA. The DNA is loaded on the top of the machine and the DNA has to travel to the bottom. The machine has two metal pins, one on the bottom and one on the top. One pin can hold either an anode or a cathode, but not both. What makes sense to connect to the bottom of the anode or the cathode to get the DNA to travel in that direction? (the top will be the opposite) What is your reasoning?
Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be transferred from another biological species easily. Their work also gave rise to the development of different recombinant proteins with therapeutic applications like insulin and growth hormone. The former was cloned using Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug produced using recombinant DNAtechnology was human insulin, developed by Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to identify and isolate the gene fragment or the gene of interest, in this case, the gene that is responsible for producing insulin. Moreover, they were able to isolate the bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase. When the DNA fragment was then placed into the bacterial DNA, it was then introduced to the host cell (E. coli) and was then…
Chapter 14 Solutions
Biology 2e
Ch. 14 - Figure 14.10 In eukaryotic cells, DNA and RNA...Ch. 14 - Figure 14.14 You isolate a cell strain in which...Ch. 14 - Figure 14.21 A fr am eshift mutation that results...Ch. 14 - If DNA of a particular species was analyzed and it...Ch. 14 - The experiments by Hershey and Chase helped...Ch. 14 - Bacterial transformation is a major concern in...Ch. 14 - DNA double helix does not have which of the...Ch. 14 - In eukaryotes, what is the DNA wrapped around?...Ch. 14 - Meselson and Stahl's experiments proved that DNA...Ch. 14 - If the sequence of the 5'-3' strand is AATGCTAC,...
Ch. 14 - How did Meselson and Stahl support Watson and...Ch. 14 - Which of the following components is not involved...Ch. 14 - Which of the following does the enzyme primase...Ch. 14 - In which direction does DNA replication take...Ch. 14 - A scientist randomly mutates the DNA of a...Ch. 14 - The ends of the linear chromosomes are maintained...Ch. 14 - Which of the following is not a true statement...Ch. 14 - During proofreading, which of the following...Ch. 14 - The initial mechanism for repairing nucleotide...Ch. 14 - A scientist creates fruit fly larvae with a...Ch. 14 - Explain Griffith's transformation experiments What...Ch. 14 - Why were radioactive sulfur and phosphorous used...Ch. 14 - When Chargaffwas performing his experiments, the...Ch. 14 - Provide a brief summary of the Sanger sequencing...Ch. 14 - Describe the structure and complementary base...Ch. 14 - Prokaryotes have a single circular chromosome...Ch. 14 - How did the scientific community learn that DNA...Ch. 14 - Imagine the Meselson and Stahl experiments had...Ch. 14 - DNA replication is bidirectional and...Ch. 14 - What are Okazaki fragments and how they are...Ch. 14 - If the rate of replication in a particular...Ch. 14 - Explain the events taking place at the replication...Ch. 14 - What is the role of a primer in DNA replication?...Ch. 14 - Quinolone antibiotics treat bacterial infections...Ch. 14 - How do the linear chromosomes in eukaryotes ensure...Ch. 14 - What is the consequence of mutation of a mismatch...Ch. 14 - An adult with a history of tanning has his genome...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A.) Chemical changes to the DNA caused by mutations can cause hazardous damage to the DNA. B.) Environmental factors such as radiation can cause damage to the DNA of the cells. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forwardIn 1950, using “blender” experiment, Hershey and Chase demonstrated that DNA, not protein, is the genetic material. They labeled proteins with radioactive phosphorus and nucleic acids with radioactive sulfur. A. Both statement is correct B. Both statement is incorrect C. Statement 1 is correct. Statement 2 is incorrect D. Statement 2 is correct. Statement 1 is incorrectarrow_forwardDiscuss the experiments Griffith, Avery and Hershey/Chase did in the early 1920-50s that helped determine DNA was the genetic stuff. What was the “take home” from each?arrow_forward
- Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.arrow_forwardThe following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloningarrow_forwardWhich of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.arrow_forward
- How could personal DNA testing be beneficial to medicine?arrow_forwardWhich of the following statements can be said about the enzyme telomerase? Select all the answers that apply. It removes RNA primers. It is highly expressed in cancer cells. It extends the length of telomeres. It exhibits reverse transcriptase activity. It shortens the ends of chromosomes.arrow_forwardDr. A. Zion wants to study the flight gene of birds. In order to study the DNA, he must first perform DNAarrow_forward
- Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forwardGriffith discovered a “transforming factor” in bacteria that conveyed infectiousness in 1928. In 1944, Avery, MacLeod and McCarty showed that the transforming factor is DNA A. Both statements are correct B. Both statements are incorrect C. First statements is correct. Second statement is incorrect D. First statements is incorrect. Second statement is correctarrow_forwardWhich of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction endonucleasec) RNA polymerased) DNA polymerasearrow_forward
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