(a) Interpretation: Fluorescence intensity versus [Cl - ] should be plotted. Concept introduction: Quenching is the process of nonradiative energy transfer from an excited species to another molecule. This requires a contact between the excited species and a quenching agent. The rate of quenching is higher when the quencher concentration is high, because of higher number of collisions during the lifetime of the excited state.

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Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213
BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

Solutions

Chapter 15, Problem 15.15QAP
Interpretation Introduction

(a)

Interpretation:

Fluorescence intensity versus [Cl-] should be plotted.

Concept introduction:

Quenching is the process of nonradiative energy transfer from an excited species to another molecule. This requires a contact between the excited species and a quenching agent. The rate of quenching is higher when the quencher concentration is high, because of higher number of collisions during the lifetime of the excited state.

Interpretation Introduction

(b)

Interpretation:

The ratio of intensity to lifetime, F-t versus [Cl-] should be plotted.

Concept introduction:

The fluorescence lifetime is the time a fluorophore exists in the excited state before returning to the ground state by emitting a photon.

Interpretation Introduction

(c)

Interpretation:

A normalization factor should be developed to correct the measured fluorescence intensity to that of the solution without quencher.

Concept introduction:

Quenching is the process of nonradiative energy transfer from an excited species to another molecule. This requires a contact between the excited species and a quenching agent. The rate of quenching is higher when the quencher concentration is high, because of higher number of collisions during the lifetime of the excited state.

Interpretation Introduction

(d)

Interpretation:

F versus [Cl-] and Fcorr versus [Cl-] should be plotted on the same graph.

Concept introduction:

Quenching is the process of nonradiative energy transfer from an excited species to another molecule. This requires a contact between the excited species and a quenching agent. The rate of quenching is higher when the quencher concentration is high, because of higher number of collisions during the lifetime of the excited state.

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