After recognition of an enhancer trap line created by P element transposition in Drosophila in which the marker gene from the enhancer trap is particularly expressed in the wing imaginal disc. To outline the procedure used to recognize the gene contiguous to the insertion site of the enhancer trap. To outline the procedure that reveals the expression pattern of the enhancer trap line and reveal the internal gene expression pattern of the contiguous gene. Introduction: Enhancer trapping is the method which allows the hijacking of an enhancer from another gene; therefore, it is useful for the identification of the enhancer. This method combines a large number of random insertion mutants with the expression of a reporter gene. The reporter gene is merged with a minimal promoter, which is insufficient to drive a detectable expression of the reporter gene used in the enhancer trapping methods. It utilizes the potential of the genomic enhancer and silencer that lie outside the construction. The expected mutation, induced by transposons, would be the insertional mutation because the additional bases are added to the original gene. The insertion of an additional base pair in the gene sequence leads to the frameshift mutation. Therefore, the mutagenesis experiment is expected to have either gain-of-function or the loss of function mutation depending on the insertion of a base pair in the transposon. The gain of function would cause overexpression of the gene inserted adjacent to the transposons, in contrast, if it is inserted into the coding region, it would show the loss of function.

Question

Want to see the full answer?

Answer 1

Question

Chapter 15, Problem 27P

Summary Introduction

To analyze:

After recognition of an enhancer trap line created by P element transposition in Drosophila in which the marker gene from the enhancer trap is particularly expressed in the wing imaginal disc.

To outline the procedure used to recognize the gene contiguous to the insertion site of the enhancer trap.

To outline the procedure that reveals the expression pattern of the enhancer trap line and reveal the internal gene expression pattern of the contiguous gene.

Introduction:

Enhancer trapping is the method which allows the hijacking of an enhancer from another gene; therefore, it is useful for the identification of the enhancer. This method combines a large number of random insertion mutants with the expression of a reporter gene. The reporter gene is merged with a minimal promoter, which is insufficient to drive a detectable expression of the reporter gene used in the enhancer trapping methods. It utilizes the potential of the genomic enhancer and silencer that lie outside the construction. The expected mutation, induced by transposons, would be the insertional mutation because the additional bases are added to the original gene. The insertion of an additional base pair in the gene sequence leads to the frameshift mutation.

Therefore, the mutagenesis experiment is expected to have either gain-of-function or the loss of function mutation depending on the insertion of a base pair in the transposon. The gain of function would cause overexpression of the gene inserted adjacent to the transposons, in contrast, if it is inserted into the coding region, it would show the loss of function.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

In Drosophila, both fushi tarazu (ftz) and engrailed (eng) genes encode homeobox transcription factors and are capable of eliciting the expression of other genes. Both genes work at about the same time during development and in the same region to specify cell fate in body segments. To discover if ftz regulates the expression of engrailed;if engrailed regulates ftz; or if both are regulated by another gene, you perform a mutant analysis. In ftz embryos (ftz/ ftz) engrailed protein is absent; in engrailed embryos (eng/eng) ftz expression is normal. What does this tell you about the regulation of these two genes—does the engrailed gene regulate ftz, or does the ftz gene regulate engrailed?

The transcription factor Pax6 is required continually during the life of a mouse (or a human) for the development of the retina. Homozygous Pax6 knockout mice die soon after birth because Pax6 protein is also required in essential organs, such as the pancreas. a) In order to study the role of Pax6 in eye development a researcher wants to generate a mouse that expresses Pax6 everywhere except in its eyes. Describe how you could construct such a mouse by floxing the gene. Is it possible to achieve the same end with a transgene? (Hint: think about using cDNA and RNAI) b) Suppose you want to create a mouse similar to that in part (a), but one where the eye cells from Pax6 function has been removed and now express a gene that specifies a green fluorescent protein (GFP). Marking the cells in this way will allow the investigators to see the shapes of the Pax6- eye cells more easily than if they did not express GFP. Diagram a Pax6 gene construct that would enable the researcher to do this…

The MAT locus allows yeast to switch mating type through a very complex mechanism. However, it has informed us a great deal about what aspects of gene expression typical to all organisms? Options: higher order changes in chromatin affect transcriptional efficiency that general transcription factors must first bind directly to histone tails and only then can they interact with their cognate binding sites that DNA methylation is involved in this silencing mechanism that SIR2 is required for all types of transcriptional repression that expression of Pol III genes provides a means of identifying active chromatin