Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 15.1, Problem 3COMQ
A bidirectional enhancer has the following sequence:
Which of the following sequences would also be a functional enhancer?
a. 5′–ACTG–3′
3′–TGAC–5′
b. 5′–TGAC–3′
3′–ACTG–5′
c. 3′–GTCA–5′
5′–CAGT–3′
d. 3′–TGAC–5′
5′–ACTG–3′
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An enhancer, located upstream from a gene, has the following sequence:
5′–GTAG–3′ 3′–CATC–5′
This enhancer is orientation-independent. Which of the following sequences also works as an enhancer?
A. 5′–CTAC–3′ 3′–GATG–5′
B. 5′–GATG–3′ 3′–CTAC–5′
C. 5′–CATC–3′ 3′–GTAG–5′ C15.
An enhancer, located upstream from a gene, has the following sequence:
5’-ACGA-3’
3’-TGCT-5’
This enhancer is orientation-independent. Which of the following sequences also works as an enhancer?
a. b.
5’-TGCT-3’ 5’-TCGT-3’
3’-ACGA-5’ 3’-AGCA-5’
you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies and explain
Chapter 15 Solutions
Genetics: Analysis and Principles
Ch. 15.1 - 1. Combinatorial control refers to the phenomenon...Ch. 15.1 - 2. A regulatory transcription factor protein...Ch. 15.1 - 3. A bidirectional enhancer has the following...Ch. 15.1 - 4. Regulatory transcription factors can be...Ch. 15.2 - 1. A chromatin-remodeling complex may
a. change...Ch. 15.2 - Prob. 2COMQCh. 15.2 - 3. Which of the following characteristics is...Ch. 15.2 - 4. Transcriptional activation of eukaryotic genes...Ch. 15.3 - How can methylation affect transcription? a. It...Ch. 15.3 - 2. The process in which completely unmethylated...
Ch. 15.4 - Prob. 1COMQCh. 15.5 - The overall goal of the ENCODE Project is a. to...Ch. 15.6 - The binding of iron regulatory protein (IRP) to...Ch. 15 - Discuss the common points of control in eukaryotic...Ch. 15 - 2. Discuss the structure and function of...Ch. 15 - 3. What is meant by the term transcription factor...Ch. 15 - What are the functions of transcriptional...Ch. 15 - 5. Is each of the following statements true or...Ch. 15 - 6. Transcription factors usually contain one or...Ch. 15 - Prob. 7CONQCh. 15 - Prob. 8CONQCh. 15 - 9. Let’s suppose a mutation in the glucocorticoid...Ch. 15 - Prob. 10CONQCh. 15 - Prob. 11CONQCh. 15 - Prob. 12CONQCh. 15 - 13. Transcription factors such as the...Ch. 15 - An enhancer, located upstream from a gene, has the...Ch. 15 - 15. The DNA-binding domain of each CREB protein...Ch. 15 - The gene that encodes the enzyme called tyrosine...Ch. 15 - Prob. 17CONQCh. 15 - 18. What is a histone variant?
Ch. 15 - Prob. 19CONQCh. 15 - 20. What is meant by the term histone code? With...Ch. 15 - Prob. 21CONQCh. 15 - Histones are thought to be displaced as RNA...Ch. 15 - 23. What is an insulator? Describe two different...Ch. 15 - 24. What is DNA methylation? When we say that DNA...Ch. 15 - Lets suppose that a vertebrate organism carries a...Ch. 15 - 26. What is a CpG island? Where would you expect...Ch. 15 - Describe how the binding of iron regulatory...Ch. 15 - 1. Briefly describe the method of chromatin...Ch. 15 - Researchers can isolate a sample of cells, such as...Ch. 15 - Prob. 3EQCh. 15 - Prob. 4EQCh. 15 - Prob. 5EQCh. 15 - 6. As described in Chapter 21, an electrophoretic...Ch. 15 - Prob. 7EQCh. 15 - 1. Explain how DNA methylation could be used to...Ch. 15 - 2. Enhancers can occur almost anywhere in DNA and...
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- Binding of _______ to _______ in DNA can increase the rate of transcription of specific genes. a. activators; repressors c. repressors; operators b. activators; enhancers d. repressors; enhancersarrow_forwardThrough alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcriptionarrow_forwardEnhancers: a. are always located upstream of the TATA sequence b. are always within 200 bp of the TATA sequence c. are responsible for time and tissue specific transcription d. All the above are true.arrow_forward
- An enhancer is a _____________ that ___________ the rate of transcription. a. trans-acting factor, increases b. trans-acting factor, decreases c. cis-acting element, increases d. cis-acting element, decreasesarrow_forwardCompare and contrast the role of promoter and operators with enhancers and repressors.arrow_forwardWhat would be the effect of moving the insulator shown in Figure 17.8 to a position between enhancer II and the promoter for gene B?arrow_forward
- You are studying a new gene that is expressed within the adipose tissue of humans during the development of diabetes. Specifically, you want to learn why gene expression levels are very high in those people who develop diabetes. What DNA sequence would you concentrate on? Group of answer choices A specific response elements B the TATA box C enhancer sequences D promoter proximal elements E metal response elementsarrow_forwardWhat is an enhancer? What do these sequences contain bound to it? Do enhancers have to be near promoters?arrow_forwardSigma (σ) factor is needed during which stage(s) of transcription? a. Initiation c. Termination b. Elongation d. All of the abovearrow_forward
- a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region. b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.arrow_forwardWhat will most likely happen when the -35 element and pribnow box are mutated? a. The sigma factor will not be able to bind to TBP. b. The rate of transcription will increase. c. RNA Polymerase II will not be able to bind to the promoter region. d.arrow_forwardThe effect that cAMP has on CAP is that a. it causes LacI to bind a promoter. b. it causes CAP to stabilize RNA polymerase binding of a promoter. c. it causes TrpR to bind a promoter. d. it causes RNA polymerase to not bind a promoter.arrow_forward
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