A. The location of silencer and enhancer. B. The reason behind the absence of silencer in the pancreatic cell. D. The reason behind no gene expression in kidney cell. Introduction: A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

Question

Your friend has discovered that the same human promoter is responsible for producing two different proteins. In Kidney cells it is responsible for the production of protein A while in Brain cells it is responsible for the production of Protein B. Your friend has concluded that this promoter must be controlling two different genes. Do you agree or disagree with your friend's conclusion? Explain why or why not. Be sure to describe the molecular events to support your answer.

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Question

Chapter 15, Problem 5EQ

Summary Introduction

To review:

A. The location of silencer and enhancer.

B. The reason behind the absence of silencer in the pancreatic cell.

D. The reason behind no gene expression in kidney cell.

Introduction:

A genetic technique called promoter bashing, identifies genetic sequences and promote regulation of gene transcription. A gene which is usually expressed in pancreatic cells is selected for promoter bashing where different segments of the upstream region labeled A to D are deleted, and DNA(Deoxyribonucleic acid) is introduced into the pancreatic cells or kidney cells. The percentage of beta-galactosidase activity is measured in both pancreatic cells and kidney cells.

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