Concept explainers
(a1)
To determine: The
Introduction:
When pyruvate is converted into acetyl CoA, one carbon molecule is lost in the form of carbon dioxide from each molecule of pyruvate. In the breakdown of pyruvate, NAD+ accepts the electron and gets converted into NADH, which is used by the cell to produce ATP. In the last step of conversion, an acetyl group is accepted by CoA to produce acetyl CoA. Acetyl CoA is the molecule which transfers the carbon atoms from pyruvate to the citric acid cycle.
(a2)
To determine: The rate of reaction when the experimental preparation containing PDH complex is treated with ATP.
Introduction:
When pyruvate is converted into acetyl CoA, one carbon molecule is lost in the form of carbon dioxide from each molecule of pyruvate. In the breakdown of pyruvate, NAD+ accepts the electron and gets converted into NADH, which is used by the cell to produce ATP. In the last step of conversion, an acetyl group is accepted by CoA to produce acetyl CoA. Acetyl CoA is the molecule which transfers the carbon atoms from pyruvate to the citric acid cycle.
(a3)
To determine: The rate of reaction when the experimental preparation containing PDH complex is treated with NADH.
Introduction:
When pyruvate is converted into acetyl CoA, one carbon molecule is lost in the form of carbon dioxide from each molecule of pyruvate. In the breakdown of pyruvate, NAD+ accepts the electron and gets converted into NADH, which is used by the cell to produce ATP. In the last step of conversion, an acetyl group is accepted by CoA to produce acetyl CoA. Acetyl CoA is the molecule which transfers the carbon atoms from pyruvate to the citric acid cycle.
(b1)
To determine: The rate of reaction when the experimental preparation containing PDH complex is treated with pyruvate dehydrogenase phosphatase.
Introduction:
PDH complex is the group of three enzymes which facilitate the conversion of pyruvate into acetyl CoA. Oxidation of pyruvate causes decarboxylation of pyruvate which is also known as pyruvate dehydrogenase reaction.
(b2)
To determine: The rate of reaction when the experimental preparation containing PDH complex is treated with Ca2+
Introduction:
PDH complex is the group of three enzymes which facilitate the conversion of pyruvate into acetyl CoA. Oxidation of pyruvate causes decarboxylation of pyruvate which is also known as pyruvate dehydrogenase reaction.
(c)
To determine: The rate of reaction when the experimental preparation containing PDH complex is treated with malonate
Introduction:
Malonate inhibits the activity of succinate dehydrogenase which terminates the cycle and the intermediate succinate
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Lehninger Principles of Biochemistry (Instructor's)
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