Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 18, Problem 20P
Summary Introduction

a.

To determine:

The signal and the switch in the sex determination pathway of Drosophila.

Introduction:

It is given that sex-determination in Drosophila depends on the number of X chromosomes such that the ratio of X chromosomes to sets of autosomes is 1 in females and 0.5 in males.

Summary Introduction

b.

To describe:

The tra- and ix- mutant phenotypes in XX flies and XY flies.

Introduction:

It is given that female flies have XX chromosomes and male flies have XY chromosomes in Drosophila. The tra and ix genes play an important role in sex determination of Drosophila.

Summary Introduction

c.

To determine:

The mutant phenotype of tra- ix- null double mutants and the mutation that would be epistatic.

Introduction:

When the phenotype of one gene depends on the presence of one or more modifier genes, this phenomenon is termed as epistasis. Null mutation makes a gene completely non-functional.

Summary Introduction

d.

To determine:

The reason due to which the epistatic mutation does not play a role in the sex determination pathway as it does in the eye development pathway.

Introduction:

It is given that the epistatic mutation defined the downstream gene in the eye development pathway. This is not the case in the sex-determination pathway.

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Which of the following applies to the female sex determination in Drosophila melanogaster fruit flies? (Mark all that apply)   When the X:A ratio is 1.0, the SXL gene is transcribed during the early embryonic stages.   The SXL protein inhibits the expression of female differentiaion genes The SXL protein promotes the correct splicing of the Tra pre-mRNA so that functional Tra protein is made.   The presence of the SXL protein promotes the correct splicing of the SXL pre-mRNA during later embryonic stages to ensure to continuous production of SXL protein.  The Tra protein will stimulate the expression of male-specific genes.
A mutation occurs in the Drosophila doublesex gene that prevents Tra from binding to the dsx RNA transcript. What would be the consequences of this mutation for Dsx protein expression in males? In females?
What would be the most likely effect of inhibiting the translation of hunchback mRNA throughout a Drosophila embryo?
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