Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 18, Problem 9P
Suppose that you generated flies containing a transgene (sev-RasS17N</I>) that uses wild-type sevenless regulatory sequences to drive the expression of coding sequences encoding a dominant negative form of the Ras protein.
| a. | Would you expect these flies to have a mutant |
| b. | Suppose you used these transgenic flies in a modifier screen. For each protein in Fig. 18.6 (p. 604) state whether loss-of-function mutations in the corresponding gene could have been identified as enhancers or suppressors of the mutant phenotype. |
| c. | Answer part (b) if the flies contained a sev-RasG12V (hypermorphic Ras) transgene instead. |
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In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true:
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Chapter 18 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - a. If you were interested in the role of a...Ch. 18 - Prob. 3PCh. 18 - Flies homozygous for recessive null mutations in...Ch. 18 - In 1932, H. J. Muller suggested a genetic test to...Ch. 18 - a. Explain how you could use worms transformed...Ch. 18 - Prob. 7PCh. 18 - Sevenless is an unusual receptor protein in that...Ch. 18 - Suppose that you generated flies containing a...Ch. 18 - Drosophila researchers have collected many strains...
Ch. 18 - As an alternative to random mutagenesis,...Ch. 18 - The molecular identity of the fruit fly rugose...Ch. 18 - Prob. 13PCh. 18 - Prob. 14PCh. 18 - Prob. 15PCh. 18 - Some ts alleles are temperature sensitive during...Ch. 18 - Prob. 17PCh. 18 - Hypomorphic alleles of a pleiotropic gene...Ch. 18 - Prob. 19PCh. 18 - Prob. 20PCh. 18 - a. Explain the difference between maternal...Ch. 18 - Prob. 22PCh. 18 - Prob. 23PCh. 18 - Some genes are required both zygotically and...Ch. 18 - How would a human with a mutation in a maternal...Ch. 18 - One important demonstration that Bicoid is an...Ch. 18 - Prob. 27PCh. 18 - Prob. 28PCh. 18 - Wild-type embryos and mutant embryos lacking the...Ch. 18 - The Drosophila even-skipped eve gene has four...Ch. 18 - Prob. 31PCh. 18 - Prob. 32P
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