Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 18, Problem 10P
Drosophila researchers have collected many strains that carry a single recombinant P element containing a wild-type white gene (a P[w+] transgene) inserted into a known genomic location. These strains can be used to map the location of any mutant gene in the fly genome.
Investigators performed a testcross to map a recessive mutation rough (ro), which causes rough eyes, relative to a P[w+] element on chromosome 3. Females heterozygous for the P[w+] on one chromosome 3 and a ro− mutation on the other, homologous chromosome 3 were crossed to ro−/ro− males, and the progeny in the following list were obtained. In both the parents and the progeny, the endogenous white gene is nonfunctional—the flies have red eyes only if they contain the P[w+] transgene.
| a. | Are ro and the P[w+] linked? If so, how many map units separate them? |
| b. | The data in part (a) do not indicate on which side of the P[w+] (toward the centromere or telomere) the ro gene is located. How could the experiment be modified to reveal that information? |
| c. | Suppose you map the ro mutation to a genomic region between two different P[w+] elements that are 5000 bp apart. Describe some experimental approaches that would allow you to identify the ro gene at the molecular level. |
| d. | How could you use the DNA sequence of the ro gene to determine the function of the protein it encodes? |
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QUESTION 11
The location of five deletions have been mapped to a Drosophila chromosome, as shown in the following deletion map.
Recessive mutations a, b, c, d and e are known to be located in the same region as the deletions but the order of the
mutations on the chromosome is not known.
Drosophila Chromosome
Deletion 1
Deletion 2
Deletion 3
Deletion 4
Deletion 5
When flies homozygous for the recessive mutation are crossed with flies heterozygous for the deletions, the following
results are obtained in which "m" represents recovery of flies with a mutant phenotype and "+" represents recovery of
only flies with a wildtype phenotype. Use the data from the table below to determine the order of the genes (a-e) on the
chromosome.
Mutants
Deletions
a
b
C
d
e
1
+
+
+
+
m
2
+
m
+
m
3
m
m
m
m
4
+
+
m
+
+
5
m
+
m
m
+
What is the gene order for these five genes? Please do not separate the letters representing the mutations with
spaces or punctuation (e.g. LMNOP)
The mutations called bobbed in Drosophila result from variable reductions (deletions) in the number of amplified genes coding for rRNA. Researchers trying to maintain bobbed stocks have often documented their tendency to revert to wild type in successive generations. Propose a mechanism based on meiotic recombination which could account for this reversion phenomenon. Why would wild-type flies become more prevalent in Drosophila cultures?
A yeast geneticist irradiates haploid cells of a strain that is an adenine-requiring auxotrophic mutant, caused by mutation of the gene ade1. Millions of the irradiated cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic, 25% adenine-requiring auxotrophsa. Explain the difference between these two types of results.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predict from crossing a prototroph of type 2 by the original ade1auxotroph?
Chapter 18 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - a. If you were interested in the role of a...Ch. 18 - Prob. 3PCh. 18 - Flies homozygous for recessive null mutations in...Ch. 18 - In 1932, H. J. Muller suggested a genetic test to...Ch. 18 - a. Explain how you could use worms transformed...Ch. 18 - Prob. 7PCh. 18 - Sevenless is an unusual receptor protein in that...Ch. 18 - Suppose that you generated flies containing a...Ch. 18 - Drosophila researchers have collected many strains...
Ch. 18 - As an alternative to random mutagenesis,...Ch. 18 - The molecular identity of the fruit fly rugose...Ch. 18 - Prob. 13PCh. 18 - Prob. 14PCh. 18 - Prob. 15PCh. 18 - Some ts alleles are temperature sensitive during...Ch. 18 - Prob. 17PCh. 18 - Hypomorphic alleles of a pleiotropic gene...Ch. 18 - Prob. 19PCh. 18 - Prob. 20PCh. 18 - a. Explain the difference between maternal...Ch. 18 - Prob. 22PCh. 18 - Prob. 23PCh. 18 - Some genes are required both zygotically and...Ch. 18 - How would a human with a mutation in a maternal...Ch. 18 - One important demonstration that Bicoid is an...Ch. 18 - Prob. 27PCh. 18 - Prob. 28PCh. 18 - Wild-type embryos and mutant embryos lacking the...Ch. 18 - The Drosophila even-skipped eve gene has four...Ch. 18 - Prob. 31PCh. 18 - Prob. 32P
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