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Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 18, Problem 24P
Some genes are required both zygotically and maternally. One experimental approach to studying such genes relies on the existence of ovoD , a dominant female sterile mutation of the ovo gene, which is located near the middle of the acrocentric Drosophila X chromosome. Females that are ovoD /ovo+ are sterile; ovoD</I>–containing germ-line cells cannot produce eggs.
a. | Mutations in gene X are recessive lethals, so homozygotes for these mutations do not develop into adults. Explain how researchers could use the ovoD mutation in a mitotic recombination experiment to determine (i) whether or not females might supply the RNA or protein product of gene X to the eggs they make in their ovaries, and (ii) whether this maternally supplied product is needed for proper development of their progeny. Where in the genome would gene X need to be located for this approach to work? |
b. | The ovoD mutant gene has been cloned, so DNA for this mutant gene is available. How could you use this cloned DNA to determine whether any embryonic lethal mutation located anywhere in the genome was an allele of a maternal effect gene? |
c. | Regardless of its chromosomal location, how could you distinguish in such an experiment whether the gene in question was a maternal effect gene, as opposed to a gene whose product is needed for oogenesis in the female? |
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Students have asked these similar questions
In drosophila, a recessive mutation (m-) of a maternal effect gene results in an abnormalphenotype wherein homozygous (m-m-) females produce eggs that cannot support embryonicdevelopment. Homozygous (m-m-) males, however, can still produce viable sperm. Using m+ todenote a normal gene, determine the genotypes and phenotypes of the F1s produce by a crossbetween a heterozygous female and a recessive male. From the offspring, backcross the recessivefemale with the paternal strain. What are the genotypes and phenotypes of the F2s?
In Drosophila subobscura, the presence of a recessive gene called grandchildless (gs) causes the offspring of homozygous females, but not those of homozygous males, to be sterile. Can you offer an explanation as to why females and not males are affected by the mutant gene?
Certain genes determine sex, fertility, and viability in Drosophila, and mutations in these genes affect the two sexes differently. Classify
the descriptions below based on their occurrence in XY or XX flies. Some descriptions may be used in both categories.
The expressed form of Dsx
activates maleness genes and
represses femaleness genes.
XY
XX
Null mutations in the dsx
gene result in an intersex
phenotype.
Loss-of-function mutations
in the tra gene have no
effect on phenotype.
Sxl is expressed in early
embryogenesis from the
establishment promoter.
Flies that produce Fru-M in
brain cells from a transgene
perform a mating dance and
specifically court females.
Loss-of-function mutations in
the fru gene result in flies who
court both males and females.
Sxl regulates splicing of
its own RNA to produce a
functional protein.
The expressed form of Dsx
activates femaleness genes
and represses maleness
genes.
Loss-of-function mutations
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Chapter 18 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - a. If you were interested in the role of a...Ch. 18 - Prob. 3PCh. 18 - Flies homozygous for recessive null mutations in...Ch. 18 - In 1932, H. J. Muller suggested a genetic test to...Ch. 18 - a. Explain how you could use worms transformed...Ch. 18 - Prob. 7PCh. 18 - Sevenless is an unusual receptor protein in that...Ch. 18 - Suppose that you generated flies containing a...Ch. 18 - Drosophila researchers have collected many strains...
Ch. 18 - As an alternative to random mutagenesis,...Ch. 18 - The molecular identity of the fruit fly rugose...Ch. 18 - Prob. 13PCh. 18 - Prob. 14PCh. 18 - Prob. 15PCh. 18 - Some ts alleles are temperature sensitive during...Ch. 18 - Prob. 17PCh. 18 - Hypomorphic alleles of a pleiotropic gene...Ch. 18 - Prob. 19PCh. 18 - Prob. 20PCh. 18 - a. Explain the difference between maternal...Ch. 18 - Prob. 22PCh. 18 - Prob. 23PCh. 18 - Some genes are required both zygotically and...Ch. 18 - How would a human with a mutation in a maternal...Ch. 18 - One important demonstration that Bicoid is an...Ch. 18 - Prob. 27PCh. 18 - Prob. 28PCh. 18 - Wild-type embryos and mutant embryos lacking the...Ch. 18 - The Drosophila even-skipped eve gene has four...Ch. 18 - Prob. 31PCh. 18 - Prob. 32P
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