Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 18, Problem 6TY
In the CRISPR-Cas technology used for mutating genes, what is(are) the function(s) of the s2.RNA?
- to bind to the target gene
- to bind to Cas9
- to cause a double-strand break in the target gene
- all of the above
- both a and b
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What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.
Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool?
A. Knocking out one or more gene
B. Activating expression of a gene
C. Suppressing expression of a gene
D. All of the above
CRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however, but instead adapted from a system that already exists in nature. Describe the native biological function of CRISPR-Cas9.
Chapter 18 Solutions
Principles of Biology
Ch. 18.1 - In the procedure shown in this figure, has the...Ch. 18.1 - Refer back to Figure 9.16. Why are primers needed...Ch. 18.1 - Prob. 2CCCh. 18.1 - Prob. 3CCCh. 18.1 - Prob. 4CCCh. 18.1 - Prob. 2BCCh. 18.1 - Prob. 1TYKCh. 18.1 - Prob. 2TYKCh. 18.1 - Prob. 3TYKCh. 18.2 - Prob. 1CC
Ch. 18.2 - Prob. 2CCCh. 18.2 - Prob. 1TYKCh. 18.2 - Prob. 2TYKCh. 18.2 - Prob. 3CCCh. 18.3 - Prob. 1TYKCh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 1BCCh. 18.4 - The sizes of eukaryotic genomes vary because more...Ch. 18.4 - The members of a gene family are called paralogs....Ch. 18.5 - Prob. 1CCCh. 18.5 - Based on their mechanism of movement, which type...Ch. 18.5 - Prob. 1TYKCh. 18.5 - A segment of DNA that moves via an RNA...Ch. 18 - Prob. 1TYCh. 18 - Prob. 2TYCh. 18 - Lets suppose you followed the protocols described...Ch. 18 - Prob. 4TYCh. 18 - Lets suppose you want to clone a gene that has...Ch. 18 - In the CRISPR-Cas technology used for mutating...Ch. 18 - Prob. 7TYCh. 18 - Prob. 8TYCh. 18 - Prob. 9TYCh. 18 - Prob. 10TYCh. 18 - Draw the structure of a dideoxyribonucleotide...Ch. 18 - Prob. 2CCQCh. 18 - Prob. 3CCQCh. 18 - Identify and discuss three important advances that...Ch. 18 - Prob. 2CBQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- explain what CRISPR/cas13 is and how is it different from CRISPR/cas9, the gene editing toolsarrow_forwardWe have isolated a mutant that changes transcription of many mRNAs, we can use to determine if there are changes to mRNA levels A. microarrays/Sequencing technologies B. Sanger sequencing C. western blot D. all of the abovearrow_forwardWhich of the following components are needed for the adaptationphase of the CRISPR-Cas system?a. crRNA and Cas1b. crRNA and Cas2c. crRNA and Cas9d. Cas1 and Cas2arrow_forward
- If a researcher wanted to utilize CRISPR/Cas9 to target a gene of interest in their experimental system (i.e. Drosophila) which of the following would they customize for this purpose Group of answer choices Guiding region of sgRNA None of the above, CRISPR can only function in bacterial cells Cas9 enzyme Scaffold region of sgRNAarrow_forwardOne concern about using genetically-modified organisms is that many of the methods used to create them introduce into the genome DNA from a different species (i.e. foreign DNA). Which of the following methods has the lowest potential of introducing foreign DNA into the genome?A. A gene knockout in mouse using homologous recombination in ES cellsB. Introduction of a P element vector into the Drosophila germ-lineC. Deletion mutations introduced by CRISPR/Cas9D. Microinjection of a transgene into a mouse pronucleusarrow_forwardExplain How Single-nucleotide mutations can be introduced into the genome using an engineered CRISPR-Cas9 system.arrow_forward
- Which of the following is NOT a true statement about CRISPR/Cas9?A. CRISPR/Cas9 is simpler to design than other gene editing technologies.B. CRISPR/Cas9 is derived from a bacterial adaptive immune defense system.C. CRISPR/Cas9 is more efficient than other gene editing technologies.D. CRISPR/Cas9 uses an RNA guide to edit a gene.E. Delivery of CRISPR/Cas9 is easier than delivery of other gene editing technologies.arrow_forwardWhich of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzymearrow_forwardResearchers create a recombinant DNA molecule in which the coding sequence for GFP is inserted downstream of the enhancer/promoter for mouse rhodopsin. Then this DNA is used to to make a trangenic mouse. This is an example of a .... Group of answer choices Expression Vector Positional Clone Reporter Genearrow_forward
- CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct? The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence. The CRISPR part recognises the DNA target sequence and Cas9 cuts it.arrow_forwardDescribe how a point mutation in a eukaryotic gene could be corrected using the CRISPR/Cas9 editing system.arrow_forwardAttached is a cartoon model of the CRISPR system, with 3 critical components labeled. A. What is the identity of each component? B. What is the function of component a? C. What is the function of component c?arrow_forward
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