Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 18.2, Problem 2TYK
Summary Introduction
Introduction:
DNA microarray is used to monitor expression of thousands of genes simultaneously. It is a collection of single stranded DNA spots with a known gene attached to a solid surface made of silica, glass or plastics. Each spot contains multiple copies of the particular gene.
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Chapter 18 Solutions
Principles of Biology
Ch. 18.1 - In the procedure shown in this figure, has the...Ch. 18.1 - Refer back to Figure 9.16. Why are primers needed...Ch. 18.1 - Prob. 2CCCh. 18.1 - Prob. 3CCCh. 18.1 - Prob. 4CCCh. 18.1 - Prob. 2BCCh. 18.1 - Prob. 1TYKCh. 18.1 - Prob. 2TYKCh. 18.1 - Prob. 3TYKCh. 18.2 - Prob. 1CC
Ch. 18.2 - Prob. 2CCCh. 18.2 - Prob. 1TYKCh. 18.2 - Prob. 2TYKCh. 18.2 - Prob. 3CCCh. 18.3 - Prob. 1TYKCh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 1BCCh. 18.4 - The sizes of eukaryotic genomes vary because more...Ch. 18.4 - The members of a gene family are called paralogs....Ch. 18.5 - Prob. 1CCCh. 18.5 - Based on their mechanism of movement, which type...Ch. 18.5 - Prob. 1TYKCh. 18.5 - A segment of DNA that moves via an RNA...Ch. 18 - Prob. 1TYCh. 18 - Prob. 2TYCh. 18 - Lets suppose you followed the protocols described...Ch. 18 - Prob. 4TYCh. 18 - Lets suppose you want to clone a gene that has...Ch. 18 - In the CRISPR-Cas technology used for mutating...Ch. 18 - Prob. 7TYCh. 18 - Prob. 8TYCh. 18 - Prob. 9TYCh. 18 - Prob. 10TYCh. 18 - Draw the structure of a dideoxyribonucleotide...Ch. 18 - Prob. 2CCQCh. 18 - Prob. 3CCQCh. 18 - Identify and discuss three important advances that...Ch. 18 - Prob. 2CBQ
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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardDiscuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell Therapyarrow_forwardWhat is vector? Discuss 2 vectors that are commonly used in DNA recombinant technology.arrow_forward
- What is the difference between cloning and genetic engineering? What is CRISPR and how does it work What is recombinant DNA? What is a plasmid What is a PCR and what does it do? List an ethical consideration when it comes to cloning or genetic engineering.arrow_forwardThe complete genetic information of a person is isolated from some of her cells, cut with restriction enzymes, and the resulting fragments inserted into cloning vectors. Next, the recombinant DNA molecules are introduced into bacteria for cloning purposes. This describes the procedure for creating a(n) ____. nucleotide library genetic library mRNA library DNA library gene libraryarrow_forwardLabel the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)arrow_forward
- What are the advantages of doing DNA sequencing?arrow_forwardWhich is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardDescribe how gel electrophoresis is used to analyze DNAarrow_forward
- Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencingarrow_forwardWhich of the following is not true about microarrays? Group of answer choices A microarray is a glass slide with single-stranded DNA attached to it. A microarray is covered in spots, where each spot represents a different sequence that will be assessed in the experiment. A microarray can assess hundreds of thousands of variants simultaneously. The same microarray slide can be used either for DNA microarrays or RNA microarraysarrow_forwardWhich of the following would you need to clone a human gene? Choose all that apply A restriction enzyme Human DNA A donor goat nucleus An enucleated egg A surrogate mother DNA ligase A plasmid vector (or other type of DNA vector, e.g. phage, BAC)arrow_forward
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