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(a)
To determine: Whether the statement, “The rate of glucose consumption per cell is approximately the same after time t as before.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E. coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(b)
To determine: Whether the statement, “The rate of lactose consumption is higher after time t than before.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(c)
To determine: Whether the statement, “The intracellular cAMP level is lower after time t than before.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(d)
To determine: Whether the statement, “Most of the lac operator sites have lac repressor proteins bound to them.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(e)
To determine: Whether the statement, “Most of the catabolite activator proteins exist as CAP-cAMP complexes.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(f)
To determine: Whether the statement, “Most of the lac repressor proteins exist as repressor-glucose complexes.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(g)
To determine: Whether the statement, “The rate of transcription of the lac operon is greater after time t than before.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
(h)
To determine: Whether the statement, “The lac operon has both CAP and the repressor protein bound to it.” is true of the cells transferred to the medium L (L), true of the cells transferred to the medium G (G), true of the cells transferred to the medium B (B) or true of none of the cells (N).
Introduction: A culture of E.coli cells growing on medium B containing both lactose and glucose is taken. At time t, 33% of the cells are transferred to medium L, which contains lactose but not glucose; 33% of the cells are transferred to medium G, which contains glucose but not lactose; and the rest 33% of the cells are left in medium B.
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Chapter 20 Solutions
Becker's World of the Cell (9th Edition)
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