Concept explainers
(a)
To determine: Three possible explanations why the mutant form of mitotic cyclin is not degraded at the onset of anaphase.
Introduction: Usually mitotic cyclin, a protein, is selectively degraded at the onset of anaphase. Recombinant DNA techniques have been used to create a mutant form of the protein. It is seen that this protein is not degraded at the onset of anaphase.
(b)
To determine: Experiments that could be carried out to distinguish among the different explanations why the mutant form of mitotic cyclin is not degraded at the onset of anaphase.
Introduction: Usually mitotic cyclin, a protein, is selectively degraded at the onset of anaphase. Recombinant DNA techniques have been used to create a mutant form of the protein. It is seen that this protein is not degraded at the onset of anaphase.
(c)
To determine: The kind of protein in which mutation has occurred such that the results are altered.
Introduction: Usually mitotic cyclin, a protein, is selectively degraded at the onset of anaphase. Recombinant DNA techniques have been used to create a mutant form of the protein. It is seen that this protein is not degraded at the onset of anaphase. A mutation was seen in a second protein which also prevents degradation of mitotic cyclin.
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Becker's World of the Cell (9th Edition)
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