(a)
To explain: The way in which testosterone and hydrocortisone are able to selectively influence the production of two different proteins in the same tissue.
Introduction: Steroid hormones are hormones which increase the expression of specific genes in selected target cell types. Testosterone is an example of a steroid hormone. It increases the production of the protein α2-microglobulin in the liver. Hydrocortisone is also an example of a steroid hormone. It increases the production of the enzyme tyrosine aminotransferase in the liver.
(b)
To determine: How the treatment of the liver with puromycin or α-amanitin prior to the administration of testosterone or hydrocortisone will affect the actions of testosterone and hydrocortisone.
Introduction: Steroid hormones are hormones which increase the expression of specific genes in selected target cell types. Testosterone is an example of a steroid hormone. It increases the production of the protein α2-microglobulin in the liver. Hydrocortisone is also an example of a steroid hormone. It increases the production of the enzyme tyrosine aminotransferase in the liver.
(c)
To determine: The effect of testosterone and hydrocortisone that will be expected in cells containing altered receptors due to a domain-swap experiment.
Introduction: Steroid hormones are hormones which increase the expression of specific genes in selected target cell types. Testosterone is an example of a steroid hormone. It increases the production of the protein α2-microglobulin in the liver. Hydrocortisone is also an example of a steroid hormone. It increases the production of the enzyme tyrosine aminotransferase in the liver.
(d)
To determine: The effect that testosterone and hydrocortisone will have in a case where the testosterone response element is substituted for a glucocorticoid response element.
Introduction: Steroid hormones are hormones which increase the expression of specific genes in selected target cell types. Testosterone is an example of a steroid hormone. It increases the production of the protein α2-microglobulin in the liver. Hydrocortisone is also an example of a steroid hormone. It increases the production of the enzyme tyrosine aminotransferase in the liver.
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Chapter 20 Solutions
Becker's World of the Cell (9th Edition)
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning