Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 22.2, Problem 2COMQ
Summary Introduction
Introduction:
Molecular pharming is a significant technique that is used for the production of medically important proteins in the mammary glands of livestock such as, sheep, cattle, and pigs. The milk produced by transgenic livestock includes some useful proteins which, are beneficial to humans. A crucial advantage of this is there are certain proteins that are more likely to function properly when produced in mammals due to certain specific covalent modifications that do not occur in bacteria.
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Gene expression in bacteria is controlled by the operon model.
a) Why is the operon model important?
b) Why is the trp operon known as a repressible operon?
A mutation occurs in the lac operon operator site such that LacI can no longer bind to the operator site. The effect that this mutation has on the cell is that
a. the activity of the lac operon will not be affected.
b. the cell can no longer convert lactose to glucose.
c. the expression of the lac genes cannot be turned off.
d. the expression of the lac genes cannot be turned on.
Which of the following is true of operons?a. They consist of a coordinated set of genes, all of which are regulated as a single unit.b. They may be inducible or repressible.c. Only bacteria and archaea utilize operons.d. All of the above are true.
Chapter 22 Solutions
Genetics: Analysis and Principles
Ch. 22.1 - 1. Which of the following uses of microorganisms...Ch. 22.1 - Prob. 2COMQCh. 22.1 - Prob. 3COMQCh. 22.2 - When a cloned gene is inserted into a noncritical...Ch. 22.2 - Prob. 2COMQCh. 22.3 - Prob. 1COMQCh. 22.3 - Prob. 2COMQCh. 22.4 - Prob. 1COMQCh. 22.4 - Prob. 2COMQCh. 22.5 - A means of introducing a cloned gene into cells...
Ch. 22.5 - 2. Which of the following best describes the...Ch. 22 - 1. What is a recombinant microorganism? Discuss...Ch. 22 - Prob. 2CONQCh. 22 - 3. What is bioremediation? What is the difference...Ch. 22 - Prob. 4CONQCh. 22 - Prob. 5CONQCh. 22 - Prob. 6CONQCh. 22 - 7. What is a transgenic organism? Describe three...Ch. 22 - Prob. 8CONQCh. 22 - Explain the difference between gene modification...Ch. 22 - As described inChapter 5, not all inherited traits...Ch. 22 - Prob. 11CONQCh. 22 - 12. Discuss the concerns that some people have...Ch. 22 - Prob. 1EQCh. 22 - 2. Bacillus thuringiensis makes toxins that kill...Ch. 22 - Prob. 3EQCh. 22 - Prob. 4EQCh. 22 - Prob. 5EQCh. 22 - What is a gene knockout? Is an animal or plant...Ch. 22 - Prob. 7EQCh. 22 - Evidence [see P. G. Shiels, A. J. Kind, K. H....Ch. 22 - Prob. 9EQCh. 22 - 10. What is reproductive cloning? Are identical...Ch. 22 - Researchers have identified a gene in humans that...Ch. 22 - Treatment of adenosine deaminase (ADA) deficiency...Ch. 22 - Several research studies are under way that...Ch. 22 - Prob. 1QSDCCh. 22 - 2. A commercially available strain of P....Ch. 22 - Prob. 3QSDC
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- Which of the following statements uses the term homologous correctly?A. The two X chromosomes in female mammalian cells are homologousto each other.B. The α-tubulin gene in Saccharomyces cerevisiae is homologousto the α-tubulin gene in Arabidopsis thaliana.C. The promoter of the lac operon is homologous to the promoterof the trp operon.D. The lacY genes of E. coli and K. pneumoniae are approximately60% homologous to each other.arrow_forwarda. What is the function of operons in bacterial gene regulation? b. Describe how a bacterial operon can be regulated by repressible proteins (such as the tryptophan operon). Include in your description both the “on” and “off” states of the operon. Key elements of your diagram should include: Co-repressor, Genes, mRNA, Operator, Operon, Promoter, Repressor, RNA polymerase.arrow_forwardWhat strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cellsarrow_forward
- The presence of glucose in the medium will lead to: a) increased cAMP concentrations and reduction of lac operon gene expression b) increased cAMP concentrations and promotion of lac operon gene expression c) reduced cAMP concentrations and reduction of lac operon gene expression d) reduced cAMP concentrations and promotion of lac operon gene expressionarrow_forwardWhich of the following statements about gene regulation is false? a. An operon contains DNA sequences that regulates its own expression. b. The operator region can only be bound by an activator protein. c. Transcription factors can affect the binding of RNA polymerase to the promoter. d. A repressor is a transcription factor that hinders gene transcription.arrow_forwardWhat is an operon ? a. A series of genes controlled by the same operator b. A series of genes on the same mRNA molecule c. A series of genes that all have the same function d. A series of genes all regulated by the same repressorarrow_forward
- What is the role of a substrate that controls a repressible operon? a. increase the production of inactive repressor proteins b. bind to the repressor protein and activate it c. bind to the repressor protein and deactivate it d. bind to the promoter region and decrease the affinity of RNA polymerase for the promoterarrow_forwardIn the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________. a) the inducer cannot bind trpO, so operon gene transcription occurs b) the active repressor cannot bind trpO, so operon gene transcription is attenuated c)the active repressor binds tpO, so operon gene transcription is always repressed d) the repressor binds the corepressor, and operon gene transcription occurs.arrow_forwardThe regulator protein that acts on a negative repressible operon is synthesized as a. an active activator. c. an active repressor. b. an inactive activator. d. an inactive repressor.arrow_forward
- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forwardWhat would occur if the repressor of an inducible operon weremutated so it could not bind the operator?(A) irreversible binding of the repressor to the promoter(B) reduced transcription of the operon’s genes(C) buildup of a substrate for the pathway controlled by theoperon(D) continuous transcription of the operon’s genesarrow_forwardHow do repressors reduce transcription of an operon? A) recruit RNA polymerase to promoters B) block the ability of RNA polymerase to bind a promoter C) break phosphodiester bonds in the DNA D) inhibit the activity of the ribosomearrow_forward
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