Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 23, Problem 2TY
Summary Introduction

Introduction:

Gram staining is the one of the most common procedures used in the field of microbiology to stain a culture of microorganisms. Depending on the results of this test bacteria can be classified as gram positive and gram negative. After the staining id done gram positive bacteria will be visible in purple and gram negative bacteria in pink under the observation of a microscope.

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A patient comes into a hospital and has what appears to be an infection. Your microscope is broken so staining is not an option. Design an experiment to determine if the infection is caused by gram positive or gram negative bacteria.
You are working in a clinical lab and culture a sample that grows on blood agar plates. When you Gram stain the culture, you see purple, spherical cells in grape-like clusters. Which of the following are correct terms that you could use to classify what you have found? (Choose all that apply.) Group of answer choices Vibriococci Staphylococci Bacteria Archaea Gram negative Gram positive
Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)?     Staining (to increase magnification).     Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation).     Add immersion oil (to increase resolution).     Add a cover slip (to reduce contamination).     The viewing chamber needs to be flushed of air (to create a vacuum).
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