The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique. Introduction: FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Answer 1

Question

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Answer 2

Question

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Answer 3

Question

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Answer 4

Question

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Answer 5

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

Answer 6

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

Answer 7

Chapter 25.7, Problem 1TQ

Summary Introduction

To review:

The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 25.1 - Prob. 1TQ Ch. 25.4 - Prob. 1TQ Ch. 25.4 - Prob. 2TQ Ch. 25.4 - Prob. 3TQ Ch. 25.4 - Prob. 4TQ Ch. 25.5 - Prob. 1TQ Ch. 25.6 - Prob. 1TQ Ch. 25.6 - Prob. 2TQ Ch. 25.6 - Prob. 3TQ Ch. 25.6 - Prob. 4TQ

Solution Summary: The author describes the FRET/beta-lactamase technique used to identify chemical inhibitors of type II, IV or VI bacterial secretion systems.

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