Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 26, Problem 13EQ

Another way to study the role of proteins (e.g., transcription factors) that function in development is to microinject the mRNA that encodes a protein, or the purified protein itself, into an oocyte or embryo, and then determine how this affects the subsequent development of the embryo, larva, and adult. For example, if Bicoid protein is injected into the posterior region of an oocyte, the resulting embryo will develop into a larva that has anterior structures at both ends. Based on your understanding of the function of each developmental gene, what would be the predicted phenotype if the following proteins or mRNAs were injected into normal oocytes?

A. Nanos mRNA injected into the anterior end of an oocyte

B. Antp protein injected into the posterior end of an embryo

C. Toll mRNA injected into the dorsal side of an early embryo

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Maternal and zygotic mRNAs regulate embryonic development by determining major body axes. Two of the these mRNAs code for the proteins ____ (of maternal origin) and (of zygotic origin), which are morphogens that accumulate at the end of the egg that will become the anterior end of the embryo, whereas two other mRNAs code for the morphogens ____ (maternal origin) and ____ (zygotic origin), which accumulate at the opposite end of the egg and determine the posterior end of the embryo. The dorso-ventral axis of the embryo is established by two morphogens coded for by maternal mRNAs:  (1) ____, which is transcribed in the egg nucleus and accumulates in a crescent on the side of the egg that will become the dorsal surface; and (2) ____, which is transported after fertilization into blastodermal cells that will become the ventral surface, and in these cells activates the zygotic gene ____, which produces a protein that diffuses to the blatodermal cells at the opposite side of the egg and…
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Chapter 26 Solutions

Genetics: Analysis and Principles

Ch. 26.4 - Prob. 1COMQCh. 26.4 - Prob. 2COMQCh. 26.5 - 1. A key event that initially determines female or...Ch. 26.5 - Prob. 2COMQCh. 26 - 1.  What four types of cellular processes must...Ch. 26 - Prob. 2CONQCh. 26 - Prob. 3CONQCh. 26 - 4. Which of the following statement(s) is/are true...Ch. 26 - Discuss the morphological differences between the...Ch. 26 - Prob. 6CONQCh. 26 - Explain what a morphogen is, and describe how it...Ch. 26 - 8.  What is positional information? Discuss three...Ch. 26 - Prob. 9CONQCh. 26 - Prob. 10CONQCh. 26 - 11. Describe the function of the Bicoid protein....Ch. 26 - With regard to development, what are the roles of...Ch. 26 - Discuss the role of homeotic genes in development....Ch. 26 - Describe the molecular features of the homeobox...Ch. 26 - What would you predict to be the phenotype of...Ch. 26 - Prob. 16CONQCh. 26 - If a mutation in a homeotic gene produced the...Ch. 26 - 18. Explain how loss-of-function mutations in the...Ch. 26 - What is the difference between a maternal-effect...Ch. 26 - Prob. 20CONQCh. 26 - Prob. 21CONQCh. 26 - Prob. 22CONQCh. 26 - 23. Discuss the similarities and differences...Ch. 26 - 24. What is cell differentiation? Discuss the role...Ch. 26 - Prob. 25CONQCh. 26 - What is a totipotent cell? In each of the...Ch. 26 - 27. What is a meristem? Explain the role of...Ch. 26 - Prob. 28CONQCh. 26 - Predict the phenotypic consequences of each of the...Ch. 26 - 30. Explain how alternative splicing affects sex...Ch. 26 - Prob. 1EQCh. 26 - Compare and contrast the experimental advantages...Ch. 26 - 3. What is meant by the term cell fate? What is a...Ch. 26 - 4. Explain why a cell lineage diagram is necessary...Ch. 26 - Explain the rationale behind the use of the bag of...Ch. 26 - Prob. 6EQCh. 26 - Take a look at question 2 in More Genetic TIPS...Ch. 26 - All of the homeotic genes inDrosophilahave been...Ch. 26 - Prob. 9EQCh. 26 - wo techniques commonly used to study the...Ch. 26 - Prob. 11EQCh. 26 - Prob. 12EQCh. 26 - 13. Another way to study the role of proteins...Ch. 26 - 14. Why have geneticists used reverse genetics to...Ch. 26 - Prob. 1QSDCCh. 26 - Prob. 2QSDCCh. 26 - Prob. 3QSDC
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