Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 26, Problem 25CQ
Summary Introduction

To explain: The reason why the insertion of 5 bp DNA at -50 position of a eukaryotic gene decreases the rate of RNA polymerase II transcription initiation to a greater extent than inserting 10 bp at the same site.

Introduction: In the transcription process, the nucleotide sequences of a gene are transcribed into mRNA sequences, which code for the proteins. There are sequences in the gene that determine the start site (upstream) and the following sequence is the downstream. These are the promoter regions. RNA polymerase enzyme binds to the promoter sequences thus, initiating the transcription process.

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