Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 26, Problem 1MTE
Summary Introduction

To determine: How the vector that is engineered to include a reporter gene flanked by a splice acceptor sequence and a polyadenylation signal is used to reveal patterns of gene expression in mammalian cells.

Introduction: Gene trapping is a method of insertional mutagenesis that is designed specifically for the disruption of a gene function. It includes random integration of a reporter gene, referred to as entrapment vector into the genome. It is used to monitor the activity of a gene.

Summary Introduction

To determine: The reason why gene trapping method depends on the size of a gene’s introns and how the trapped gene is identified.

Introduction: Gene trapping is a method of insertional mutagenesis that is designed specifically for the disruption of a gene function. It includes random integration of a reporter gene referred to as entrapment vector into the genome. It is used to monitor the activity of a gene.

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