An equation for mRNA phosphorolysis reaction. Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

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Answer 1

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

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Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

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Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

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Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

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Answer 5

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 6

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 7

Chapter 26, Problem 2E

(a)

Summary Introduction

To write: An equation for mRNA phosphorolysis reaction.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 8

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 9

(b)

Summary Introduction

To explain: How PNPase accomplish the reverse of phosphorolysis reaction in vitro and how it differs from the reaction carried out by RNA polymerase.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 10

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 11

(c)

Summary Introduction

To explain: How the property of PNPase having a binding site for long ribonucleotides is an advantage for its primary activity in vivo.

Introduction: Polynucleotide phosphorylase (PNPase) is an exoribonuclease that is involved in the degradation and processing of mRNA. It catalyzes 3ʹ-5ʹ phosphorolysis reaction using inorganic phosphate for degradation and nucleotide diphosphate (NDP) for polymerization process.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 26 - Prob. 1E Ch. 26 - 2. The bacterial enzyme polynucleotide...Ch. 26 - Prob. 3E Ch. 26 - Prob. 4E Ch. 26 - Prob. 5E Ch. 26 - Prob. 6E Ch. 26 - Prob. 7E Ch. 26 - Prob. 8E Ch. 26 - Prob. 9E Ch. 26 - 10. Certain E. coli bacteriophages encode a...

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