Concept explainers
Interpretation:
The double-stranded DNA sequence code for metenkephalin has to be predicted.
Concept Introduction:
Base pairing in DNA: The two strands of the DNA double helix run in the opposite directions- one in 5’ to 3’ direction and other from 3’ to 5’ direction. The hydrogen bonding between two strands enhances the stability of the DNA; where the alignment of hydrophobic nitrogenous bases in the interior and hydrophilic phosphate and sugar groups on the exterior also enhance the stability. Adenine and thymine gives a pair forming two hydrogen bonds and cytosine and guanine gives rise to another pair forming three hydrogen bonds.
Codon: A sequence of three ribonucleotides in the mRNA chain that codes for a specific amino acid; also a three-
Genetic code: The sequence of nucleotides, coded in triplets (codons) in mRNA that determines the sequence of amino acids in protein synthesis.
Illustrated relationships are:
DNA informational strand: 5’ ATG CCA GTA GGC CAC TTG TCA 3’
DNA Template strand: 3’ TAC GGT CAT CCG GTG AAC AGT 5’
mRNA: 5’ AUG CCA GUA GGC CAC UUG UCA 3’
protein: Met Pro Val Gly His Leu Ser
Notice: 5’ end of the mRNA strand codes for the N-terminal amino acid, whereas the 3’ end of the mRNA strand codes for the C-terminal amino acid. Proteins are always written N-terminal to C-terminal, reading left to right.
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EBK FUNDAMENTALS OF GENERAL, ORGANIC, A
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- Consider a three-base sequence in the template of DNA: 5' . . . 123 . . .3', in which 1, 2, and 3 refer to the relative positions of deoxyribonucleotides.Comment on the probable effect on the resulting protein if the following point mutations (one-base substitutions) occurred.(a) changing one purine for another in position 1(b) changing one pyrimidine for another in position 2(c) changing a purine to a pyrimidine in position 2(d) changing one purine for another in position 3arrow_forwardDescribe the functions of the following proteins during DNA breaks and repair: (i) Ku70 (ii) Uracil DNA glycosylasearrow_forwardDiscuss the Pharmacokinetics of morphine sulfate using the correct sequence you have chosen in question number one.arrow_forward
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