BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
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Chapter 29, Problem 11P
Interpretation Introduction
Interpretation:
The reason for making DNA radioactive needs to be suggested.
Concept introduction:
DNA endonuclease is an enzyme which can cut the DNA strand by breaking the phosphodiester bond between two nucleotides. It will generate one 3’OH and one 5’ phosphoryl group at the end of broken strands.
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Pstl.
EcoRI
Origin of
replication
(ori)
Ampicillin Tetracycline
resistance resistance
(Amp) (Tet")
pBR322
(4,361 bp)
BamHI
Pvull
Sall
Recombinant
Plasmid DNA
Bacterial cell contains...
No plasmid DNA
pBR322 (no insert)
Recombinant plasmid
00,000.
Host DNA
Transformation
of E. coli cells
+AMP plate
pBR322
Figure 7-5
+TET plate
Based on the recombinant plasmid growth pattern (bottom row of blue table), which of
the depicted plasmid's restriction sites was used to prepare this sample? Explain how
you can tell.
Chapter 29 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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