Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 3, Problem 28P
Interpretation Introduction
Interpretation:
In the given experiment, the observation needs to be explained, if the given protein does not contain any disulfide bond and if it contains a single disulfide bond. An experiment to identify the cysteine residue containing the disulfide bond needs to be explained.
Concept introduction:
2-D electrophoresis is a technique that is used to analyze proteins. Here mixture of proteins is separated based on two properties in two dimensions of the same gel. Normally, in the first dimension proteins are separated according to the isoelectric point and then the proteins are separated in a perpendicular dimension based on their mass.
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Question:-
Based on the figure below, predict what peptide bond could be the substrate of each protease(The bond marked in blue is where hydrolysis occurs, choose 2 peptides per protease type)
Chymotrypsin:_________ Trypsin:_________ Elastase:_________
1. SR−SG 2. SF−SG 3. SK−SG 4. SA−SG 5. SV−SG 6. SM−SG
Simple problem-solving: Imagine you did a Bradford assay, and measured the absorbance of a set of protein standards and two unknowns. You repeated each measurement three times, and got the following readings in the table below.
Concentration (micrograms/ml)
A595 -
trial 1
A595- trial 2
A595- trial 2
average A595
A595 of sample minus A595 of blank
0
1.501
1.446
1.447
1.465
0
2
1.624
1.558
1.559
1.580
0.115
5
1.731
1.749
1.712
1.731
0.266
10
1.901
1.838
1.892
1.877
0.412
15
2.161
2.108
2.228
2.166
0.701
18
2.231
2.277
2.319
2.276
0.811
unknown 1 - D1
1.717
1.713
1.644
1.691
0.226
unknown 2 - D1
1.668
1.649
1.656
1.658
0.193
concentration of unknown 1
concentration of unknown 2
Calculate the concentration of the unknowns and answer the following question:
Did you need to do any manipulations before applying the…
Help?
Please explain salting out in protein purification.
Chapter 3 Solutions
Biochemistry
Ch. 3 - Prob. 1PCh. 3 - Prob. 2PCh. 3 - Prob. 3PCh. 3 - Prob. 4PCh. 3 - Prob. 5PCh. 3 - Prob. 6PCh. 3 - Prob. 7PCh. 3 - Prob. 8PCh. 3 - Prob. 9PCh. 3 - Prob. 10P
Ch. 3 - Prob. 11PCh. 3 - Prob. 12PCh. 3 - Prob. 13PCh. 3 - Prob. 14PCh. 3 - Prob. 15PCh. 3 - Prob. 16PCh. 3 - Prob. 17PCh. 3 - Prob. 18PCh. 3 - Prob. 19PCh. 3 - Prob. 20PCh. 3 - Prob. 21PCh. 3 - Prob. 22PCh. 3 - Prob. 23PCh. 3 - Prob. 24PCh. 3 - Prob. 25PCh. 3 - Prob. 26PCh. 3 - Prob. 27PCh. 3 - Prob. 28PCh. 3 - Prob. 29PCh. 3 - Prob. 30PCh. 3 - Prob. 31P
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