Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 3, Problem 12P
Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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